Programmed conversion of hypertrophic chondrocytes into osteoblasts and marrow adipocytes within zebrafish bones

ELife
Dion GiovannoneJ Gage Crump

Abstract

Much of the vertebrate skeleton develops from cartilage templates that are progressively remodeled into bone. Lineage tracing studies in mouse suggest that chondrocytes within these templates persist and become osteoblasts, yet the underlying mechanisms of this process and whether chondrocytes can generate other derivatives remain unclear. We find that zebrafish cartilages undergo extensive remodeling and vascularization during juvenile stages to generate fat-filled bones. Growth plate chondrocytes marked by sox10 and col2a1a contribute to osteoblasts, marrow adipocytes, and mesenchymal cells within adult bones. At the edge of the hypertrophic zone, chondrocytes re-enter the cell cycle and express leptin receptor (lepr), suggesting conversion into progenitors. Further, mutation of matrix metalloproteinase 9 (mmp9) results in delayed growth plate remodeling and fewer marrow adipocytes. Our data support Mmp9-dependent growth plate remodeling and conversion of chondrocytes into osteoblasts and marrow adipocytes as conserved features of bony vertebrates.

References

Nov 1, 1977·The Journal of Bone and Joint Surgery. British Volume·K von der Mark, H von der Mark
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R MayneE J Miller
Dec 22, 1975·Calcified Tissue Research·Y ShimomuraF Suzuki
Apr 1, 1994·Differentiation; Research in Biological Diversity·K HeermeierJ Schmidt
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·N HoggardJ G Mercer
Aug 26, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·P BiancoR Cancedda
Aug 9, 2002·Developmental Biology·Nathan D Lawson, Brant M Weinstein
Nov 5, 2002·Journal of Cell Science·Claudia HegertJürgen Rohwedel
Jul 23, 2004·PLoS Biology·Justin Gage CrumpCharles B Kimmel
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Masaki InadaStephen M Krane
Oct 28, 2005·Development·Suk-Won JinDidier Y R Stainier
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·Andrea Page-McCawZena Werb
Oct 17, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kristen M KwanChi-Bin Chien
Apr 23, 2009·Biological Reviews of the Cambridge Philosophical Society·P Eckhard Witten, Ann Huysseune
May 28, 2010·Genesis : the Journal of Genetics and Development·April DeLaurierCharles B Kimmel
May 17, 2011·Developmental Cell·Franziska KnopfGilbert Weidinger
Jul 5, 2011·Developmental Biology·Rodney M Dale, Jacek Topczewski
Mar 7, 2012·BMC Evolutionary Biology·B Frank EamesJohn H Postlethwait
Apr 28, 2012·Nature·Vikas Gupta, Kenneth D Poss
Nov 17, 2012·PloS One·Erika KagueShannon Fisher
Jan 31, 2013·Nature Biotechnology·Woong Y HwangJ Keith Joung
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Liu YangKathryn S E Cheah
Nov 25, 2014·Nature Cell Biology·Noriaki OnoHenry M Kronenberg
Sep 6, 2015·Journal of Dental Research·Y JingJ Q Feng
Mar 30, 2017·Disease Models & Mechanisms·James E N Minchin, John F Rawls
Sep 27, 2018·Nature·Shawon DebnathMatthew B Greenblatt

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Citations

Aug 15, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rong HuangGurpreet S Baht
Mar 13, 2020·Development·Maxwell A SerowokyFrancesca V Mariani
Jul 15, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Zhijia TanKathryn S E Cheah
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Sophia Ka Yan MaDanny Chan
Jan 30, 2020·The FEBS Journal·Jason K B Nguyen, B Frank Eames
Aug 28, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brian P HeubelPierre Le Pabic
Jan 24, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kristin DietrichFranziska Knopf
Jul 30, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Camilo Riquelme-GuzmánTatiana Sandoval-Guzmán

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Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

GraphPad Prism
Fiji
Adobe Photoshop
ZEN

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