Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

Frontiers in Microbiology
Anica DadwalTulasi Satyanarayana

Abstract

Lignocellulosic biomass is a renewable and sustainable energy source. Cellulases are the enzymes that cleave β-1, 4-glycosidic linkages in cellulose to liberate sugars that can be fermented to ethanol, butanol, and other products. Low enzyme activity and yield, and thermostability are, however, some of the limitations posing hurdles in saccharification of lignocellulosic residues. Recent advancements in synthetic and systems biology have generated immense interest in metabolic and genetic engineering that has led to the development of sustainable technology for saccharification of lignocellulosics in the last couple of decades. There have been several attempts in applying genetic engineering in the production of a repertoire of cellulases at a low cost with a high biomass saccharification. A diverse range of cellulases are produced by different microbes, some of which are being engineered to evolve robust cellulases. This review summarizes various successful genetic engineering strategies employed for improving cellulase kinetics and cellulolytic efficiency.

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Citations

Jul 29, 2020·Journal of Biomolecular Structure & Dynamics·Sukanya KonarPradip Kr Ghorai
Jan 9, 2021·Journal of Biomolecular Structure & Dynamics·Anica DadwalTulasi Satyanarayana
Feb 9, 2021·Biotechnology and Applied Biochemistry·Isabela Victorino da Silva AmattoHamilton Cabral
Apr 25, 2021·Biotechnology for Biofuels·Peng NingJianming Yang
Aug 10, 2021·International Journal of Biological Macromolecules·Anica DadwalTulasi Satyanarayana

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Methods Mentioned

BETA
targeted modifications
glycosylation
genetic modification
genetic modifications
PCR
transglycosylation

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