Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand-gated channels, and showing the potential to play a pivotal role in drug discovery and biomedical research. Unfortunately, the cell suspension assays to which early systems were limited cannot recreate biologically relevant cellular environments, or capture higher order aspects of synaptic physiology and network dynamics. In vivo patch clamp electrophysiology has the potential to yield more biologically complex information and be especially useful in reverse engineering the molecular and cellular mechanisms of single-cell and network neuronal computation, while capturing important aspects of human disease mechanisms and possible therapeutic strategies. Unfortunately, it is a difficult procedure with a steep learning curve, which has restricted dissemination of the technique. Luckily, in vivo patch clamp electrophysiology seems particularly amenable to robotic automation. In this review, we document the development of automated patch clamp technology, from early systems based on mult...Continue Reading
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Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches
A Chip-Based Biosensor for the Functional Analysis of Single Ion Channels We thank E. Ermanntraut, L. Giovangrandi, T. Wohland, A. Brecht, M. Köhler, C. Bieri, D. Stamou, and R. Hovius for advice. This work was supported by the Swiss National Science Foundation (Priority Program for Biotechnology) and by an interdepartmental grant of the Swiss Federal Institute of Technology Lausanne (EPFL, Project Microtechnique 96)
In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain
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Automated tight seal electrophysiology for assessing the potential hERG liability of pharmaceutical compounds
Development of a novel automated ion channel recording method using "inside-out" whole-cell membranes
Estimation of both the potency and efficacy of alpha7 nAChR agonists from single-concentration responses
Population patch clamp improves data consistency and success rates in the measurement of ionic currents
Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development
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A novel silicon patch-clamp chip permits high-fidelity recording of ion channel activity from functionally defined neurons
Introduction of a modular automated voltage-clamp platform and its correlation with manual human Ether-à-go-go related gene voltage-clamp data
Pain-related deactivation of medial prefrontal cortical neurons involves mGluR1 and GABA(A) receptors
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State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
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Development of a high-throughput electrophysiological assay for the human ether-à-go-go related potassium channel hERG
A comparison of the performance and application differences between manual and automated patch-clamp techniques
A finite rate of innovation algorithm for fast and accurate spike detection from two-photon calcium imaging
Free-standing kinked nanowire transistor probes for targeted intracellular recording in three dimensions
ACQ4: an open-source software platform for data acquisition and analysis in neurophysiology research
Evaluating state dependence and subtype selectivity of calcium channel modulators in automated electrophysiology assays
Characterizing human stem cell-derived sensory neurons at the single-cell level reveals their ion channel expression and utility in pain research
Using physiologically-based pharmacokinetic-guided "body-on-a-chip" systems to predict mammalian response to drug and chemical exposure
In vivo measurement of cell-type-specific synaptic connectivity and synaptic transmission in layer 2/3 mouse barrel cortex
A novel selective and orally bioavailable Nav 1.8 channel blocker, PF-01247324, attenuates nociception and sensory neuron excitability
Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7
Endogenous opioids contribute to insensitivity to pain in humans and mice lacking sodium channel Nav1.7
Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp
Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes
Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module
Automated Electrophysiological and Pharmacological Evaluation of Human Pluripotent Stem Cell-Derived Cardiomyocytes
Subtype-Selective Small Molecule Inhibitors Reveal a Fundamental Role for Nav1.7 in Nociceptor Electrogenesis, Axonal Conduction and Presynaptic Release
Biophysical and Pharmacological Characterization of Nav1.9 Voltage Dependent Sodium Channels Stably Expressed in HEK-293 Cells
Three Small-Receptive-Field Ganglion Cells in the Mouse Retina Are Distinctly Tuned to Size, Speed, and Object Motion
Two dimensional electrophysiological characterization of human pluripotent stem cell-derived cardiomyocyte system
Automated and manual patch clamp data of human induced pluripotent stem cell-derived dopaminergic neurons
Using automated patch clamp electrophysiology platforms in pain-related ion channel research: insights from industry and academia
Advances in two photon scanning and scanless microscopy technologies for functional neural circuit imaging
Closed-Loop Real-Time Imaging Enables Fully Automated Cell-Targeted Patch-Clamp Neural Recording In Vivo
Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels
An update on the advancing high-throughput screening techniques for patch clamp-based ion channel screens: implications for drug discovery
Accounting for variability in ion current recordings using a mathematical model of artefacts in voltage-clamp experiments
A Temperature-Controlled Patch Clamp Platform Demonstrated on Jurkat T Lymphocytes and Human Induced Pluripotent Stem Cell-Derived Neurons
Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms
Mirroring Action Potentials: Label-Free, Accurate, and Noninvasive Electrophysiological Recordings of Human-Derived Cardiomyocytes.
Endogenous Bioelectrics in Development, Cancer, and Regeneration: Drugs and Bioelectronic Devices as Electroceuticals for Regenerative Medicine
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