Progress in automating patch clamp cellular physiology

Brain and Neuroscience Advances
Luca A Annecchino, Simon R Schultz


Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand-gated channels, and showing the potential to play a pivotal role in drug discovery and biomedical research. Unfortunately, the cell suspension assays to which early systems were limited cannot recreate biologically relevant cellular environments, or capture higher order aspects of synaptic physiology and network dynamics. In vivo patch clamp electrophysiology has the potential to yield more biologically complex information and be especially useful in reverse engineering the molecular and cellular mechanisms of single-cell and network neuronal computation, while capturing important aspects of human disease mechanisms and possible therapeutic strategies. Unfortunately, it is a difficult procedure with a steep learning curve, which has restricted dissemination of the technique. Luckily, in vivo patch clamp electrophysiology seems particularly amenable to robotic automation. In this review, we document the development of automated patch clamp technology, from early systems based on mult...Continue Reading


Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J EberwineP Coleman
Jan 1, 1990·Biophysical Journal·D P WellisM Mazzanti
Oct 1, 1990·Biophysical Journal·M Mazzanti, L J DeFelice
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillFred J Sigworth
Feb 11, 1994·Science·M ChalfieD C Prasher
Aug 1, 1995·Trends in Genetics : TIG·J Pines
Nov 15, 1996·Cell·P MombaertsR Axel
Nov 14, 1997·Journal of Neurophysiology·B JagadeeshD Ferster
Apr 18, 2002·Biosensors & Bioelectronics·Kathryn G KlemicFred J Sigworth
May 23, 2002·Biophysical Journal·Niels FertigJ C Behrends
Jul 12, 2002·Current Gene Therapy·K Lundstrom
Jul 24, 2002·Pflügers Archiv : European journal of physiology·Troy W MargrieBert Sakmann
Apr 15, 2003·Medical & Biological Engineering & Computing·A StettW Nisch
Jul 12, 2003·Journal of Biomolecular Screening·Kirk SchroederJennings Worley
Sep 16, 2003·Neuron·Troy W MargrieMichael Brecht
Feb 3, 2004·Physical Review Letters·R Alexander KaulPeter Fromherz
Apr 20, 2004·Assay and Drug Development Technologies·Laszlo KissKirk Schroeder
Apr 20, 2004·Assay and Drug Development Technologies·Jia XuLei Wu
Apr 20, 2004·Assay and Drug Development Technologies·Xiaobo Wang, Min Li
Dec 4, 2004·Pflügers Archiv : European journal of physiology·Kathryn G KlemicFred J Sigworth
Dec 8, 2004·Progress in Brain Research·Jaap van PeltGer J A Ramakers
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tanjew DittgenPavel Osten
Jan 22, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel A WagenaarSteve M Potter
Jan 27, 2005·Assay and Drug Development Technologies·Huimin TaoAndrea Ghetti
Jun 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Cristian Ionescu-ZanettiLuke P Lee
Jul 5, 2005·Nature Neuroscience·Hiroshi IshikaneMasao Tachibana
Oct 20, 2005·Journal of Biomolecular Screening·Dmitry V VasilyevM R Bowlby
Jan 24, 2006·The Journal of Pain : Official Journal of the American Pain Society·John D Markman, Robert H Dworkin
Apr 6, 2006·Pflügers Archiv : European journal of physiology·Dmitry V VasilyevM R Bowlby
Jun 9, 2006·Journal of Biomolecular Screening·Alan FinkelJames Costantin
Nov 10, 2006·Journal of Biomolecular Screening·Victoria H JohnDerek J Trezise
Nov 15, 2006·Lab on a Chip·Chihchen Chen, Albert Folch
Dec 15, 2006·Nature·James J CoxC Geoffrey Woods
Dec 21, 2006·Advances in Pharmacology·S J Enna, Kenneth E McCarson
Dec 29, 2006·Small·Andrea BrüggemannNiels Fertig
Mar 6, 2007·Pflügers Archiv : European journal of physiology·Thomas Nevian, Fritjof Helmchen
Mar 31, 2007·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Diana Conte CamerinoJean-François Desaphy
Apr 5, 2007·Nature Protocols·Shoji KomaiTroy W Margrie
Oct 24, 2007·Trends in Neurosciences·Sulayman D Dib-HajjStephen G Waxman
Jan 19, 2008·Nature·Julia KnablHanns Ulrich Zeilhofer
Mar 22, 2008·Nature Reviews. Drug Discovery·J DunlopRobert Arias
Apr 23, 2008·Current Protocols in Neuroscience·Michael R DeWeese
Oct 2, 2008·Methods in Molecular Biology·Michael FejtlAlbrecht Lepple-Wienhues
Jan 20, 2009·Combinatorial Chemistry & High Throughput Screening·Chris MathesYi Liu
Feb 7, 2009·Nature Protocols·Carol J MilliganDavid J Beech
May 16, 2009·Nature Protocols·Benjamin JudkewitzMichael Häusser
Jun 25, 2009·Methods in Molecular Biology·Kenneth A JonesCharles H Large
Jun 26, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Simon R SchultzMichael Häusser
Sep 4, 2009·Methods in Molecular Biology·William P Janzen, Paul Bernasconi
Sep 24, 2009·Journal of Biomolecular Screening·Dmitry V VasilyevM R Bowlby
Dec 2, 2009·Frontiers in Molecular Neuroscience·Daniel F GilbertTimothy I Webb
Feb 4, 2010·The Journal of Physiology·Mark EstacionStephen G Waxman
Apr 2, 2010·Lab on a Chip·Kum Cheong TangLevent Yobas
Jul 8, 2010·Biomaterials·Thomas SordelNathalie Picollet-D'hahan
Sep 11, 2010·Advanced Materials·John M NagarahJames R Heath
Feb 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jacob Menzler, Günther Zeck
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rodrigo PerinHenry Markram
Sep 2, 2011·Journal of Neurophysiology·Guangchen Ji, Volker Neugebauer
May 9, 2012·Nature Methods·Suhasa B KodandaramaiahCraig R Forest
May 12, 2012·Assay and Drug Development Technologies·C Ian SpencerCristian Ionescu-Zanetti
Aug 31, 2012·Frontiers in Genetics·Shenfeng QiuKai Wang
Oct 30, 2012·Journal of Pharmacological and Toxicological Methods·Daniel J GillieClaire Townsend
Jun 19, 2013·Scientific Reports·Ryuji KawanoShoji Takeuchi
Jun 25, 2013·Pain·Ru-Rong JiMaiken Nedergaard
Mar 1, 2007·IEEE Transactions on Biomedical Circuits and Systems·J N Y AzizP L Carlen
Jul 19, 2013·Journal of Neural Engineering·Jon OñativiaPier Luigi Dragotti
Aug 21, 2013·Journal of Biomolecular Screening·Jean-Marie ChambardMichel Partiseti
Sep 3, 2013·Neuroscience Letters·Garth T WhitesideJeffrey D Kennedy
Feb 14, 2014·Frontiers in Neuroinformatics·Luke CampagnolaPaul B Manis
Mar 4, 2014·Assay and Drug Development Technologies·Yuri A KuryshevGlenn E Kirsch
May 17, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gareth T YoungEdward B Stevens
Jun 21, 2014·Nature Reviews. Drug Discovery·Ru-Rong JiYong-Jing Gao
Jul 26, 2014·Bioorganic & Medicinal Chemistry Letters·Sharan BagalNigel A Swain
Jan 9, 2015·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Renaud SchuckSimon R Schultz
Jan 23, 2015·Frontiers in Neuroscience·Marie Engelene J ObienUrs Frey
Apr 10, 2015·Journal of Neurophysiology·Nikita PakEdward S Boyden
Jun 19, 2015·Journal of Neurophysiology·Niraj S DesaiDaniel Johnston
Aug 26, 2015·Journal of Biomolecular Screening·Edward S A Humphries, Caroline Dart
Nov 7, 2015·Nature Reviews. Drug Discovery·Gerald W Zamponi
Nov 12, 2015·Channels·Alison ObergrussbergerClemens Möller
Dec 15, 2015·Acta Pharmacologica Sinica·Hai-bo YuXiao-liang Wang
Dec 17, 2015·Cell Reports·Jean-Sébastien JouhanneauJames F A Poulet
Dec 17, 2015·BMC Pharmacology & Toxicology·Yuji HaraguchiTatsuya Shimizu
Dec 23, 2015·Nature Biotechnology·Cathryn R CadwellAndreas S Tolias
Dec 23, 2015·Scientific Reports·Brian LongHanchuan Peng
Dec 25, 2015·Journal of Laboratory Automation·Alison ObergrussbergerNiels Fertig
Jan 7, 2016·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Renaud SchuckSimon R Schultz
Mar 19, 2016·Assay and Drug Development Technologies·Chris ChambersJuha Kammonen
Mar 31, 2016·Science Signaling·Kristopher T KahleGuy A Rouleau
May 29, 2016·Trends in Pharmacological Sciences·Gareth R TibbsPeter A Goldstein
Jul 8, 2016·Journal of Neurophysiology·Qiuyu Wu 吴秋雨Alexander A Chubykin
Oct 12, 2016·Scientific Reports·I KolbCraig R Forest
Nov 5, 2016·Science·Ru-Rong JiYu-Qiu Zhang
Nov 23, 2016·Scientific Reports·Hanna Keren, Shimon Marom
Jan 20, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jason Jacoby, Gregory W Schwartz
Jun 9, 2017·Journal of Neurophysiology·W A StoyCraig R Forest
Nov 14, 2017·Journal of Neural Engineering·Renaud SchuckSimon R Schultz
Nov 28, 2017·Methods in Molecular Biology·Rikiya WatanabeHiroyuki Noji
Jan 4, 2018·ELife·Suhasa B KodandaramaiahCraig R Forest


May 26, 2020·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Chon Lok LeiGary R Mirams
Aug 1, 2020·Electrophoresis·Erin A Henslee
Apr 11, 2019·Journal of Neural Engineering·I KolbCraig R Forest
Mar 6, 2020·Frontiers in Bioengineering and Biotechnology·Giovanni MelleFrancesco De Angelis
Dec 31, 2020·International Journal of Molecular Sciences·Ekaterina S PotekhinaDmitry S Bilan
Feb 18, 2021·Expert Opinion on Drug Metabolism & Toxicology·Anke M Tukker, Remco H S Westerink
Nov 25, 2020·Journal of Cardiovascular Pharmacology·Arie O Verkerk, Ronald Wilders
Sep 24, 2018·Cell Systems·John SelbergMarco Rolandi
Aug 21, 2020·Neurobiology of Pain·Sampurna ChakrabartiEwan St John Smith

Methods Mentioned

fluorescence microscopy

Related Concepts

Biomedical Research
Bone Plates
Patch-Clamp Techniques
Tissue Engineering
Molecular Dynamics
Biological Neural Networks
Ligand-Gated Ion Channels

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.