Progress in automating patch clamp cellular physiology

Brain and Neuroscience Advances
Luca A Annecchino, Simon R Schultz

Abstract

Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand-gated channels, and showing the potential to play a pivotal role in drug discovery and biomedical research. Unfortunately, the cell suspension assays to which early systems were limited cannot recreate biologically relevant cellular environments, or capture higher order aspects of synaptic physiology and network dynamics. In vivo patch clamp electrophysiology has the potential to yield more biologically complex information and be especially useful in reverse engineering the molecular and cellular mechanisms of single-cell and network neuronal computation, while capturing important aspects of human disease mechanisms and possible therapeutic strategies. Unfortunately, it is a difficult procedure with a steep learning curve, which has restricted dissemination of the technique. Luckily, in vivo patch clamp electrophysiology seems particularly amenable to robotic automation. In this review, we document the development of automated patch clamp technology, from early systems based on mult...Continue Reading

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Methods Mentioned

BETA
transgenic
fluorescence microscopy
chip
chips
transfecting
transfection
scRNA-seq

Related Concepts

Biomedical Research
Bone Plates
Environment
Research
Disseminated
Patch-Clamp Techniques
Tissue Engineering
Molecular Dynamics
Biological Neural Networks
Ligand-Gated Ion Channels

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