Progress in isolation technic of islets of Langerhans for transplantation

An improved technique is described which yields up to 6,000 isolated islets of Langerhans from the pancreas of rats. The quotients for insulin/amylase and insulin/protein were estimated in vitro. For in vivo testing isologous transplantations of islets were performed. Several parameters such as total body-weight, blood sugar levels, insulin i.s., glucose-tolerance and glucose-assimilation tests demonstrated that the islets still retained their normal function despite the isolation procedure.