Progress in the identification of interaction sites on the periplasmic maltose binding protein from E coli

Biochimie
P MartineauF A Quiocho

Abstract

The periplasmic maltose binding protein (MBP) is required for the high affinity transport of maltose and maltodextrins and for chemotaxis towards these sugars. In these functions, MBP interacts with proteins of the cytoplasmic membrane: MalF and MalG for transport, Tar for chemotaxis. A large number of MBP mutations have been isolated by us and other laboratories. We grouped these mutations into classes depending on the interactions affected and we represented the corresponding residues on the 3-D model for MBP so as to further identify the sites of MBP interacting with the MalF-MalG complex and with the Tar protein. MBP (like the other binding proteins) is composed of 2 lobes enclosing a cleft where the substrate binds. The face of the protein opposite the cleft seems to interact neither with MalF-MalG nor with Tar. The other face, corresponding to the cleft, contains sites for interactions with MalF-MalG and Tar. These sites appear to cover both sides of the cleft and may overlap in part. The present definition of the interaction sites suggests further that MBP has different in vivo orientations when it interacts with MalF-MalG or with Tar. This work constitutes an additional step in combining the use of genetic and structura...Continue Reading

References

Oct 1, 1979·Journal of Bacteriology·C WandersmanT Ferenci
Apr 1, 1975·Journal of Bacteriology·G L Hazelbauer
Jan 30, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·F A Quiocho
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J B WeissP J Bassford
Jan 1, 1987·Annual Review of Genetics·H A Shuman
Jan 1, 1986·Journal of Bacteriology·M D Manson, M Kossmann
Jan 1, 1986·Annual Review of Biochemistry·G F Ames
Aug 15, 1974·European Journal of Biochemistry·O Kellermann, S Szmelcman
Aug 2, 1984·Nature·F A Quiocho, N K Vyas

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Citations

Jan 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M H Saier
Mar 1, 1997·Protein Science : a Publication of the Protein Society·S SzmelcmanM Hofnung
Feb 6, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·C S SouzaA Balan
Aug 19, 1999·Protein Science : a Publication of the Protein Society·R B Kapust, D S Waugh
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Amy L DavidsonJue Chen
Jun 20, 1997·The Journal of Biological Chemistry·G Richarme, T D Caldas
May 1, 2004·The Journal of Biological Chemistry·Mariana I AustermuhleAmy L Davidson
Sep 1, 2010·EcoSal Plus·Amy L Davidson, Frances Joan D Alvarez

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