Progress in transduction of cerebellar Purkinje cells in vivo using viral vectors.

The Cerebellum
Hirokazu Hirai

Abstract

Expression of a foreign gene in cerebellar Purkinje cells in vivo is a powerful method for exploring the pathophysiology of the cerebellum. Although using developmental engineering many gene-modified mice have been generated, this approach is time-consuming and requires a lot of effort for crossing different lines of mice, genotyping and maintenance of animals. If a gene of interest can be transferred to and efficiently expressed in Purkinje cells of developing and mature animals, it saves much time, effort and money. Recent advances in viral vectors have markedly contributed to selective and efficient gene transfer to Purkinje cells in vivo. There are two approaches for selective gene expression in Purkinje cells: one is to take advantage of the viral tropism for Purkinje cells, which includes the tropism of adeno-associated virus and the vesicular stomatitis virus glycoprotein (VSV-G)-pseudotyped lentivirus. Another method, which might be used in combination with the first one, is utilization of a Purkinje-cell-specific promoter. Focusing mainly on these points, recent progress in viral-vector-mediated transduction of Purkinje cells in vivo is reviewed.

References

Jan 20, 1996·Human Gene Therapy·M HashimotoK Mikoshiba
Mar 10, 1997·The Journal of Cell Biology·B SodeikA Helenius
Jun 13, 1998·Human Gene Therapy·J S BartlettT J McCown
Nov 18, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·W F KaemmererW C Low
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Guangping GaoJames M Wilson
Oct 22, 2003·Progress in Neurobiology·Justyna R Sarna, Richard Hawkes
Dec 20, 2003·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Marina MataDavid J Fink
Jun 7, 2005·Expert Opinion on Biological Therapy·Shin-ichi MuramatsuKeiya Ozawa
Oct 7, 2005·BioTechniques·Rachael L NeveWilliam A Carlezon
Mar 7, 2006·Brain Research·Takashi TorashimaHirokazu Hirai
Jul 14, 2006·The European Journal of Neuroscience·Takashi TorashimaHirokazu Hirai
Dec 14, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bradford K BergesNigel W Fraser
Apr 5, 2007·Nature Protocols·Gustavo TiscorniaInder M Verma

❮ Previous
Next ❯

Citations

May 20, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nobuhiko OhnoBruce D Trapp
Aug 12, 2010·The Cerebellum·Jean-Pierre LouboutinDavid S Strayer
Dec 18, 2012·The Cerebellum·Clévio NóbregaLuís Pereira de Almeida
Dec 31, 2008·Journal of Neuroscience Methods·Masahiko TanakaNaohide Hirashima
Jul 11, 2012·The European Journal of Neuroscience·Jun NishiyamaMichisuke Yuzaki
Sep 11, 2010·Molecular and Cellular Neurosciences·Philip W J BurnetMatthew J A Wood
Sep 8, 2010·Molecular and Cellular Neurosciences·Yohei OhashiYasushi Miyashita
Oct 5, 2014·The Cerebellum·Kazuhiro NakamuraHirokazu Hirai
Aug 30, 2016·PloS One·Yoichiro ShinoharaHirokazu Hirai
Aug 6, 2008·Annual Review of Phytopathology·Juan A Díaz-Pendón, Shou-Wei Ding
Dec 24, 2019·Human Molecular Genetics·Fabiana LongoStefano Carlo Previtali
Oct 13, 2009·Neurochemical Research·Masahiko Tanaka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.