Progress toward clonable inorganic nanoparticles

Nanoscale
Thomas W NiC J Ackerson

Abstract

Pseudomonas moraviensis stanleyae was recently isolated from the roots of the selenium (Se) hyperaccumulator plant Stanleya pinnata. This bacterium tolerates normally lethal concentrations of SeO3(2-) in liquid culture, where it also produces Se nanoparticles. Structure and cellular ultrastructure of the Se nanoparticles as determined by cellular electron tomography shows the nanoparticles as intracellular, of narrow dispersity, symmetrically irregular and without any observable membrane or structured protein shell. Protein mass spectrometry of a fractionated soluble cytosolic material with selenite reducing capability identified nitrite reductase and glutathione reductase homologues as NADPH dependent candidate enzymes for the reduction of selenite to zerovalent Se nanoparticles. In vitro experiments with commercially sourced glutathione reductase revealed that the enzyme can reduce SeO3(2-) (selenite) to Se nanoparticles in an NADPH-dependent process. The disappearance of the enzyme as determined by protein assay during nanoparticle formation suggests that glutathione reductase is associated with or possibly entombed in the nanoparticles whose formation it catalyzes. Chemically dissolving the nanoparticles releases the enzyme...Continue Reading

References

Jan 1, 1995·Methods in Enzymology·M BjörnstedtA Holmgren
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·J N ChaD E Morse
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Achilleas S FrangakisWolfgang Baumeister
Mar 6, 2003·Nature Materials·Rajesh R NaikMorley O Stone
Jan 9, 2004·Applied and Environmental Microbiology·Ronald S OremlandSeamus Curran
Oct 13, 2004·Carbohydrate Research·Haizhen Huang, Xiurong Yang
Jan 18, 2005·Trends in Cell Biology·Richard McIntoshDavid Mastronarde
Sep 1, 2005·Journal of Industrial Microbiology & Biotechnology·Simon Silver, Le T Phung
Nov 25, 2005·Journal of Molecular Biology·Christopher P Mercogliano, David J DeRosier
Dec 15, 2005·Journal of the American Chemical Society·Lina A GugliottiBruce E Eaton
Feb 17, 2006·Nature Reviews. Molecular Cell Biology·Stephan NickellWolfgang Baumeister
Mar 15, 2006·Biochimica Et Biophysica Acta·Jesús M de la Fuente, Soledad Penadés
Apr 15, 2006·Science·Ben N G GiepmansRoger Y Tsien
Sep 30, 2006·Angewandte Chemie·Nils KrögerVonda C Sheppard
Dec 29, 2006·Small·Joseph M SlocikRajesh R Naik
Jan 18, 2007·FEMS Microbiology Letters·Marina BasagliaSergio Casella
Apr 3, 2007·Current Opinion in Structural Biology·Grant J Jensen, Ariane Briegel
Apr 5, 2007·Nature Protocols·Cristina V IancuGrant J Jensen
May 22, 2007·Biochemical Society Transactions·M M TomczakR R Naik
Aug 19, 2007·Journal of Structural Biology·Christopher P Mercogliano, David J DeRosier
Dec 14, 2007·Nature·Carol V RobinsonWolfgang Baumeister
Mar 19, 2008·Journal of the American Chemical Society·Chris I RichardsRobert M Dickson
Apr 5, 2008·Current Microbiology·William J Hunter, Daniel K Manter
Dec 31, 2008·Journal of Structural Biology·Elia DiestraCristina Risco
Feb 4, 2009·Current Microbiology·William J Hunter, Daniel K Manter
Feb 26, 2010·Angewandte Chemie·Chun-Long Chen, Nathaniel L Rosi
Sep 4, 2010·Current Microbiology·William J Hunter, Daniel K Manter
Oct 5, 2010·Methods in Enzymology·Christopher J AckersonJames F Hainfeld
Feb 9, 2011·Structure·Qing WangJan Löwe
Feb 18, 2011·Nature Communications·T ScopignoT Wagner
Mar 17, 2011·Nanoscale·Isabel Díez, Robin H A Ras
May 24, 2011·Applied and Environmental Microbiology·Markus LenzPhilippe F X Corvini
Oct 21, 2011·ACS Nano·Kamalesh ChaudhariThalappil Pradeep
Dec 14, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Jing YuGustavo A Carri

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Citations

Jan 7, 2015·Chemosphere·Lucian C StaicuPiet N L Lens
Dec 13, 2019·Frontiers in Microbiology·Brandi CronJulie Cosmidis
Dec 31, 2020·FEMS Microbiology Ecology·Zachary J ButzChristopher J Ackerson
Jun 23, 2020·ACS Chemical Biology·Zachary J ButzChristopher J Ackerson
Apr 18, 2021·FEMS Microbiology Ecology·Lucian C Staicu, John F Stolz
Jun 13, 2021·Journal of Inorganic Biochemistry·Lucian C Staicu, Larry L Barton
Nov 7, 2019·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Ryan A RiskowskiChristopher J Ackerson

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