Progression of LNCaP prostate tumor cells during androgen deprivation: hormone-independent growth, repression of proliferation by androgen, and role for p27Kip1 in androgen-induced cell cycle arrest

Molecular Endocrinology
John M KokontisS Liao

Abstract

The molecular mechanism of androgen-independent growth of prostate cancer after androgen ablation was explored in LNCaP cells. An androgen-dependent clonal subline of the LNCaP human prostate carcinoma cell line, LNCaP 104-S, progressed to a slow growing stage (104-R1) and then to a faster growing stage (104-R2) during more than 2 yr of continuous culture in the absence of androgen. Androgen-induced proliferation of 104-S cells is inhibited by the antiandrogen Casodex, while proliferation of 104-R1 and 104-R2 cells is unaffected by Casodex. This indicates that proliferation of 104-R1 and 104-R2 cells is not supported by low levels of androgen in the culture medium. Compared with LNCaP 104-S cells, both 104-R1 and 104-R2 cells express higher basal levels of androgen receptor (AR), and proliferation of these two cell lines is paradoxically repressed by androgen. After continuous passage in androgen-containing medium, 104-R1 cells reverted back to an androgen-dependent phenotype. The mechanism of androgenic repression of 104-R1 and 104-R2 sublines was further evaluated by examining the role of critical regulatory factors involved in the control of cell cycle progression. At concentrations that repressed growth, androgen transientl...Continue Reading

References

May 10, 1991·International Journal of Cancer. Journal International Du Cancer·T H van der KwastJ Trapman
Apr 1, 1991·Molecular and Cellular Endocrinology·A KrongradM J McPhaul
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·G J van SteenbruggeF H Schröder
Jan 1, 1990·Molecular Endocrinology·V E QuarmbyE M Wilson
Jan 1, 1989·Urological Research·G J van SteenbruggeJ Horoszewicz
Aug 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·C M SmithJ R Masters
May 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·P A BaleyT C Thompson
Oct 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·M O Joly-PharabozJ G Andre
Jul 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·M KogaB Sato
May 15, 1995·Genes & Development·C J Sherr, J M Roberts
Oct 13, 1994·The New England Journal of Medicine·W J Catalona
May 1, 1994·International Journal of Cancer. Journal International Du Cancer·H C WuL W Chung
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
Aug 16, 1996·The Journal of Biological Chemistry·L V Nazareth, N L Weigel
Oct 1, 1996·Endocrinology·P ReinikainenO A Jänne
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y UmekitaS Liao
Oct 14, 1996·Biochemical and Biophysical Research Communications·M V BlagosklonnyW S el-Deiry
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·H Y ZhauL W Chung

❮ Previous
Next ❯

Citations

Apr 8, 2010·Pharmaceutical Research·Subhajit Mukherjee, Sridhar Mani
Jan 11, 2012·Asian Journal of Andrology·Yoshito HirataKazuyuki Aihara
Dec 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jung Hwa KimSung Hee Baek
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·S YehC Chang
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·M StanbroughS P Balk
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·P GeckA M Soto
Sep 3, 2009·The Journal of Biological Chemistry·Fangming TangJialing Xiang
Feb 1, 2011·Cancer Prevention Research·Mee Young HongDavid Heber
Aug 7, 2007·Theoretical Biology & Medical Modelling·A Edward Friedman
Mar 10, 2012·PloS One·Yelena MirochnikOlga V Volpert
Feb 23, 2011·The Oncologist·Rahul Aggarwal, Charles J Ryan
Feb 26, 2009·Endocrine-related Cancer·Jason M D'AntonioJohn T Isaacs
Feb 16, 2006·Molecular and Cellular Biology·Yuting LinJialing Xiang
Aug 24, 2011·Journal of Biomedical Science·Chih-Pin ChuuLiang-Cheng Su
Jul 21, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Takashi OtsukaKazuyuki Hirano
Mar 21, 2007·Journal of Biomedical Science·Chih-Pin ChuuShutsung Liao
Aug 19, 2009·General and Comparative Endocrinology·Valérie S LangloisVance L Trudeau
Oct 1, 2003·The Prostate·Adrie van BokhovenM Scott Lucia
Mar 8, 2013·International Journal of Molecular Sciences·Hui-Ping LinChih-Pin Chuu
Jun 4, 2004·The Prostate·Xu-Bao ShiRalph W deVere White
Oct 27, 2015·The Prostate·Mark A CastanaresCatherine A Foss
Oct 6, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Gouhei TanakaKazuyuki Aihara
Dec 9, 2004·American Journal of Physiology. Endocrinology and Metabolism·Yen-Hang ChenYung-Hsi Kao
Jan 12, 2005·The Journal of Urology·R E Sobel, M D Sadar
Jan 14, 2005·American Journal of Physiology. Cell Physiology·Pei-Fang HungYung-Hsi Kao
May 10, 2007·International Journal of Cancer. Journal International Du Cancer·Thomas NeliusOlga V Volpert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.