Progression of Thyroid Carcinoma Is Promoted by the m6A Methyltransferase METTL3 Through Regulating m6 A Methylation on TCF1

OncoTargets and Therapy
Kejin WangChao Chen

Abstract

This study aims to uncover the progression of thyroid carcinoma influenced by the m6A methyltransferase METTL3 through regulating m6A methylation on TCF1 mRNA and the activated Wnt pathway. Thyroid carcinoma tissues and paracancerous ones were collected for detecting levels of METTL3 and TCF1. Potential correlation between levels of METTL3 and TCF1 was analyzed by Pearson analysis. Survival of thyroid carcinoma patients influenced by METTL3 level was assessed by Kaplan-Meier method. Regulatory effect of METTL3 on migratory ability in TPC-1 cells was examined by wound healing assay. The interaction between METTL3 with TCF1 and IGF2BP2 was verified by RNA-Binding Protein Immunoprecipitation (RIP) assay. Meanwhile, the activity of the Wnt pathway was reflected by TOP/FOP-Flash. At last, rescue experiments were conducted to clarify the involvement of TCF1 in phenotype changes of thyroid carcinoma cells that were regulated by METTL3. METTL3 and TCF1 were upregulated in thyroid carcinoma. Similarly, METTL3 was highly expressed in thyroid carcinoma cells as well. Kaplan-Meier method uncovered poor prognosis in thyroid carcinoma patients expressing a high level of METTL3. Silence of METTL3 inhibited migratory ability and Wnt activity i...Continue Reading

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Citations

Aug 29, 2020·Journal of Hematology & Oncology·Chengwu ZengHengyou Weng
Jan 24, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jing HeFucheng He
Jan 13, 2021·Cell & Bioscience·Jianzhong GuXinbing Sui
Apr 22, 2021·Biomarker Research·Yiqing CaiXin Wang
May 1, 2021·Journal of Experimental & Clinical Cancer Research : CR·Fenghua TanCan Guo
Sep 26, 2021·The Journal of Clinical Endocrinology and Metabolism·Shaojian LinXinzhi Peng
Nov 6, 2021·Frontiers in Immunology·Fangzhou TengJingcheng Dong

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Methods Mentioned

BETA
Protein
Assay
RIP
transfection
surgical resection

Software Mentioned

SPSS

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