PMID: 7538321May 1, 1995Paper

Progression to androgen insensitivity in a novel in vitro mouse model for prostate cancer

The Journal of Steroid Biochemistry and Molecular Biology
P A BaleyT C Thompson

Abstract

We have shown previously that the ras and myc oncogenes can induce poorly differentiated mouse prostate carcinomas in vivo with high frequency (greater than 90%) using inbred C57BL/6 mice in the mouse prostate reconstitution model system. To study the androgen sensitivity of these carcinomas, we have developed an in vitro model system which includes a cell line from normal urogenital sinus epithelium (CUGE) and cell lines from three ras + myc transformed mouse prostate carcinomas (RM-9, RM-1, and RM-2). CUGE cells, as well as all prostate carcinoma cell lines, were positive for cytokeratin 18 mRNA and immunoreactive to cytokeratin-specific antiserum. Two out of three of the early passage carcinoma cell lines were clonal with respect to Zipras/myc 9 retrovirus integration as determined by Southern blot analysis. Whereas significant mitogenic effects of testosterone (10 nM) were not seen in CUGE cells grown in serum-free medium, under similar conditions approx. 2-fold increases in cell number were seen in all low passage prostate carcinoma cell lines. Also, in the presence of growth inhibitory levels of suramin (50 micrograms/ml), testosterone was capable of significant growth stimulation in the carcinoma cell lines. With further...Continue Reading

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Citations

Sep 7, 2007·Cancer Immunology, Immunotherapy : CII·M-C LabartheM A Whelan
Nov 23, 2010·Cancer Immunology, Immunotherapy : CII·Huanfa YiXiang-Yang Wang
Sep 1, 2005·Angiogenesis·Xiaojun HuangElieser Gorelik
May 29, 2008·Clinical & Experimental Metastasis·N Patrick McCabeTatiana V Byzova
Nov 25, 2011·Pathology Oncology Research : POR·Andreia M RibeiroMariana P Monteiro
May 23, 2012·Oncogene·M K HerroonI Podgorski
Jan 12, 2011·Prostate Cancer and Prostatic Diseases·K L SantucciJ G Baust
Nov 5, 2011·Journal of Endourology·Vladimir MouravievChristopher Lascola
Aug 1, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Tzu G WuWilliam Robert Fleischmann
Mar 19, 1997·Journal of the National Cancer Institute·M KawakitaT L Ratliff
Apr 25, 2002·Molecular and Cellular Biology·Chengzhen RenTimothy C Thompson
Sep 10, 2009·Molecular Cancer Research : MCR·Masami WatanabeTimothy C Thompson
Dec 1, 2001·Expert Opinion on Biological Therapy·T C ThompsonD Kadmon
May 1, 2015·PloS One·Ângela MoreiraMariana P Monteiro
Jun 11, 2002·Biochemical and Biophysical Research Communications·Jian-Feng LiuDominique Ledoux
Apr 26, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·S W SukinE Aguilar-Cordova
Jul 21, 2017·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·SoHyun HanEllen Ackerstaff
Dec 2, 2017·International Journal of Molecular Sciences·Coralie GenevoisNicolas Grenier
Sep 23, 2006·International Journal of Cancer. Journal International Du Cancer·Julieann F GrantTimothy L Ratliff
Jul 9, 2009·The Prostate·Carl A PowerPamela J Russell
Jul 30, 2010·International Journal of Experimental Pathology·Andreia M RibeiroMariana P Monteiro

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