PMID: 9525642Apr 3, 1998Paper

Progression to the G1b phase of the cell cycle is required for completion of human immunodeficiency virus type 1 reverse transcription in T cells

Journal of Virology
Y D Korin, J A Zack

Abstract

Successful infection by human immunodeficiency virus type 1 (HIV-1) requires the activation of target cells. Infection of quiescent peripheral CD4 lymphocytes by HIV-1 results in incomplete, labile, reverse transcripts. In the present study, we isolated highly purified quiescent T cells and utilized the CD3/CD28 activation pathways as well as cell cycle inhibitors to further define the role of costimulation and cell cycle progression in HIV-1 reverse transcription. Activation with alphaCD3 alone resulted in cell cycle progression into only G1a and incomplete HIV-1 reverse transcription. Costimulation through the CD28 receptor and transition into G1b was required to efficiently complete the reverse transcription process. These findings have relevance to immune activation in vivo, since lymphocytes rendered anergic by a single activation signal would be nonpermissive for productive infection with HIV-1. Importantly, these data also suggest that HIV vector-based genetic transduction strategies might be successful only in target cells that transition into the G1b phase of the cell cycle.

References

May 1, 1992·Cellular Immunology·K M GilbertW O Weigle
Aug 11, 1992·Journal of Virology·F LoriR C Gallo
Jan 29, 1991·Biochemistry·D H Sorscher, M Cordeiro-Stone
Jun 15, 1990·Science·R H Schwartz
Jun 1, 1990·Immunology Today·C H JuneC B Thompson
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Jan 1, 1984·Advances in Experimental Medicine and Biology·S SpadariG Pedrali-Noy
Dec 1, 1981·Experimental Cell Research·Z DarzynkiewiczM R Melamed
Jul 1, 1994·The Journal of General Virology·E J ArtsM A Wainberg
Jun 1, 1995·Current Opinion in Immunology·D L Mueller, M K Jenkins
Jan 1, 1995·Advances in Experimental Medicine and Biology·J A Zack
Jan 1, 1995·Advances in Experimental Medicine and Biology·M StevensonM Emerman
Jan 28, 1994·Cell·C A Janeway, K Bottomly
Mar 1, 1993·European Journal of Immunology·R CostelloD Olive
Jan 28, 1994·Cell·T KishimotoS Akira
Mar 1, 1996·Science·P E FieldsF W Fitch
Apr 1, 1997·The Journal of Clinical Investigation·C A SpinaD D Richman
Jun 1, 1997·Current Opinion in Immunology·C A Chambers, J P Allison

❮ Previous
Next ❯

Citations

Dec 30, 1999·Nature Genetics·M Emerman
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L WangL Su
Jul 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·V DardalhonN Taylor
Jan 5, 2002·Journal of Virology·Ulrich KuhnJonathan C Vogel
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·M MengozziM Roederer
Jan 31, 2002·Annual Review of Medicine·Joel N BlanksonRobert F Siliciano
Aug 7, 2002·Journal of Virology·Theodore C PiersonRobert F Siliciano
Nov 28, 2002·Blood·Sara JalecoNaomi Taylor
Sep 24, 2004·Microbiology and Immunology·Midori TaruishiNaoki Yamamoto
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Annette AudigéRoberto F Speck
Jul 15, 2006·PLoS Pathogens·Kara G LassenRobert F Siliciano
Sep 22, 2006·Clinical and Vaccine Immunology : CVI·Kevin B Gurney, Christel H Uittenbogaart
Sep 16, 2006·Current Rheumatology Reports·Fons A J van de LooWim B van den Berg
Aug 12, 2009·PloS One·Francesca R Santoni de Sio, Didier Trono
Aug 21, 2010·Immunologic Research·Dimitrios N VatakisJerome A Zack
Apr 9, 2013·Current Opinion in HIV and AIDS·Marco CraveiroNaomi Taylor
May 2, 2013·Autophagy·Tania C FelizardoDaniel H Fowler
Sep 8, 2016·AIDS Research and Human Retroviruses·John Henry MorrisAnding Shen
Jun 6, 2002·Journal of Cellular Biochemistry. Supplement·Jeffrey A Medin, Daniel H Fowler
Jul 23, 2002·Journal of Virology·Yael D KorinJerome A Zack
Oct 9, 2002·Journal of Virology·Una O'DohertyMichael H Malim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.