Progressive ankylosis gene (ank) regulates osteoblast differentiation.

Cells, Tissues, Organs
Thorsten KirschJeffrey A Winkles

Abstract

The progressive ankylosis gene (ank) is a transmembrane protein that transports intracellular pyrophosphate to the extracellular milieu. Human mutations of ank lead to craniometaphyseal dysplasia, a disease which is characterized by the overgrowth of craniofacial bones and osteopenia in long bones, suggesting that ANK plays a regulatory role in osteoblast differentiation. To determine the role of ANK in osteoblast differentiation, we suppressed ANK expression in the osteoblastic MC3T3-E1 cell line using siRNA and determined the expression of osteoblastic marker genes and the transcription factors osterix and runx2. In addition, we determined the osteoblastic differentiation of bone marrow stromal cells isolated from the bone marrow of ank/ank mice, which express a truncated, nonfunctional ANK protein, or wild-type littermates. Suppression of ANK expression in MC3T3-E1 cells led to a decrease in bone marker gene expression, including alkaline phosphatase, bone sialoprotein, osteocalcin and type I collagen. In addition, osterix gene expression was decreased in ANK expression-suppressed MC3T3 cells, whereas runx2 expression was increased. Bone marrow stromal cells isolated from ank/ank mice cultured in the presence of ascorbate-2-...Continue Reading

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Citations

Feb 12, 2013·Annals of the Rheumatic Diseases·Kate Louise Eve PhillipsChristine Lyn Le Maitre
Apr 2, 2016·Current Rheumatology Reports·Elizabeth Mitton-FitzgeraldAnn K Rosenthal
Apr 7, 2017·European Journal of Endocrinology·Eveline Boudin, Wim Van Hul
Sep 8, 2009·Bone·Monika D PolewskiRobert Terkeltaub
Feb 3, 2012·The Journal of Rheumatology·Facundo Las HerasFlorence W L Tsui
Feb 27, 2010·American Journal of Medical Genetics. Part a·Allison ZajacHyun-Duck Nah

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