Progressive degeneration of nigrostriatal dopamine neurons following intrastriatal terminal lesions with 6-hydroxydopamine: a combined retrograde tracing and immunocytochemical study in the rat

Neuroscience
H Sauer, W H Oertel

Abstract

In order to develop a rodent model displaying a progressive degeneration of the dopamine neurons of the substantia nigra, we bilaterally injected the tracer substance FluoroGold into the terminal field of the nigrostriatal projection, i.e. the striatum. One week later, rats received unilateral injections of 20 micrograms 6-hydroxydopamine into one of the two striatal tracer deposits. Groups of animals were killed one, two, four, eight and 16 weeks later. Ipsilateral to the lesion there was a progressive loss of FluoroGold-labelled nigral cells, with cell counts dropping from 96% of the contralateral side at one week to 59% at two weeks, 35% at four weeks, 23% at eight weeks and down to 15% at 16 weeks. Labelled nigral neurons ipsilateral to the lesion showed a moderate to marked atrophy at all investigated time points. The number of tyrosine hydroxylase-immunoreactive cells was decreased to 83% of contralateral at one week, 39% at two weeks, 44% at four weeks, 34% at eight weeks and 52% at 16 weeks postlesion. Rhodamine fluorescence immunocytochemistry showed that the proportion of surviving ipsilateral fluorogold-labelled cells displaying immunoreactivity for tyrosine hydroxylase was 69% at one week postlesion, 51% at two week...Continue Reading

References

Jan 1, 1975·Biochemical Pharmacology·C Sachs, G Jonsson
Jun 1, 1992·Brain : a Journal of Neurology·L E AnnettS B Dunnett
Sep 1, 1992·Progress in Neuro-psychopharmacology & Biological Psychiatry·Y Kushima, H Hatanaka
Dec 1, 1992·Neurochemical Research·A CestelliI Di Liegro
Aug 6, 1990·Brain Research·I Divac, J Mogensen
Feb 5, 1990·Brain Research·M H TuszynskiF H Gage
Jan 1, 1988·Experimental Brain Research·L C TriarhouB Ghetti
Nov 1, 1988·Experimental Neurology·R L McBrideB E Smith
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L R WilliamsF H Gage
Dec 1, 1968·European Journal of Pharmacology·U Ungerstedt
Jan 1, 1980·Annual Review of Neuroscience·G Jonsson

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Citations

Oct 31, 2000·The Journal of Comparative Neurology·C L ZuchP C Bickford
Dec 13, 1997·Molecular Neurobiology·P Horellou, J Mallet
Feb 15, 2008·Acta Neuropathologica·Gloria E MeredithMarie-Francoise Chesselet
Oct 27, 2004·Cell and Tissue Research·Andreas Schober
Sep 1, 2004·Cell and Tissue Research·Peter Teismann, Jörg B Schulz
Aug 17, 2004·Cell and Tissue Research·Laurent RoybonJia-Yi Li
Sep 10, 2011·Journal of Neural Transmission·Bokyung ParkYoung J Oh
Dec 10, 2009·Neurochemical Research·Ho-Joo LeeAbesh Kumar Bhattacharjee
Aug 18, 2010·Cell Biochemistry and Biophysics·Chuanqin FangChangyue Gao
Dec 24, 2011·Neurotoxicity Research·Mohd Moshahid KhanFakhrul Islam
Jan 1, 1994·Life Sciences·A Kupsch, W H Oertel
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Rowan M BurnsteinMaeve A Caldwell
Apr 9, 2005·Experimental Neurology·Nadine P Poulton, Gillian D Muir
May 5, 2005·Experimental Neurology·Thomas DebeirRita Raisman-Vozari
May 17, 2005·Neuroscience Letters·Carmen NoelkerRichard Dodel
Dec 7, 2002·Brain Research·Maria J MartíEduard Tolosa
Nov 9, 2000·European Journal of Pharmacology·N JonkersY Michotte
Oct 14, 2003·Experimental Neurology·Timothy J CollierJeffrey H Kordower
Aug 8, 2002·Neurotoxicology·R H GwiazdaD R Smith

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