Progressive telomere dysfunction causes cytokinesis failure and leads to the accumulation of polyploid cells.

PLoS Genetics
Judit PampalonaLaura Tusell

Abstract

Most cancer cells accumulate genomic abnormalities at a remarkably rapid rate, as they are unable to maintain their chromosome structure and number. Excessively short telomeres, a known source of chromosome instability, are observed in early human-cancer lesions. Besides telomere dysfunction, it has been suggested that a transient phase of polyploidization, in most cases tetraploidization, has a causative role in cancer. Proliferation of tetraploids can gradually generate subtetraploid lineages of unstable cells that might fire the carcinogenic process by promoting further aneuploidy and genomic instability. Given the significance of telomere dysfunction and tetraploidy in the early stages of carcinogenesis, we investigated whether there is a connection between these two important promoters of chromosomal instability. We report that human mammary epithelial cells exhibiting progressive telomere dysfunction, in a pRb deficient and wild-type p53 background, fail to complete the cytoplasmatic cell division due to the persistence of chromatin bridges in the midzone. Flow cytometry together with fluorescence in situ hybridization demonstrated an accumulation of binucleated polyploid cells upon serial passaging cells. Restoration of ...Continue Reading

References

Jun 1, 1977·The Journal of Cell Biology·J M Mullins, J J Biesele
Aug 1, 1990·Mutation Research·V W Mayer, A Aguilera
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·P C GalipeauB J Reid
May 1, 1996·Cell Proliferation·G JuanZ Darzynkiewicz
Apr 10, 1997·Nature·C LengauerB Vogelstein
Jan 1, 1999·Nature·C LengauerB Vogelstein
Nov 5, 2002·Nature Reviews. Cancer·Erich A Nigg
May 15, 2003·International Journal of Radiation Biology·M MartínL Tusell
Jan 7, 2004·Nature Reviews. Molecular Cell Biology·Zuzana Storchova, David Pellman
Feb 26, 2004·The American Journal of Pathology·Alan K MeekerPedram Argani
Mar 24, 2004·Current Opinion in Cell Biology·Greenfield Sluder, Joshua J Nordberg
May 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alan K MeekerAngelo M De Marzo
Apr 15, 2005·Nature·Vassilis G GorgoulisThanos D Halazonetis
Jul 30, 2005·Genes, Chromosomes & Cancer·David SolerLaura Tusell
Aug 27, 2005·Carcinogenesis·Andrew J OlaharskiDavid A Eastmond
May 23, 2006·Cancer Letters·Carlo C Maley
Jun 3, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lan GuoYong Qian
Jul 4, 2006·Genetics·James RonaldRachel B Brem
Aug 18, 2006·Nature·Beth A A WeaverDon W Cleveland
Sep 26, 2006·Nature Cell Biology·Paola VagnarelliWilliam C Earnshaw
Oct 7, 2006·Nature·Zuzana StorchováDavid Pellman
Dec 27, 2006·Cancer Cell·Rocío SotilloRobert Benezra
Feb 28, 2007·Current Opinion in Genetics & Development·Neil J GanemDavid Pellman
Feb 5, 2008·Nature Genetics·Mariarosaria MusaròGiovanni Cenci
Sep 5, 2008·Current Protocols in Cytometry·G Juan, Z Darzynkiewicz
Nov 21, 2008·Journal of Cell Science·Zuzana Storchova, Christian Kuffer
Feb 4, 2009·Cytogenetic and Genome Research·L TusellA Genescà
Feb 11, 2009·Cell·Patrick SteigemannDaniel W Gerlich
Jun 10, 2009·Nature·Neil J GanemDavid Pellman
Sep 10, 2009·Journal of Cell Science·Liliana TorosantucciFrancesca Degrassi
Jan 21, 2010·Genes, Chromosomes & Cancer·Judit PampalonaLaura Tusell
Apr 8, 2010·Cell·Teresa DavoliTitia de Lange
Oct 6, 2010·The Journal of Clinical Investigation·Patrizia VinciguerraAlan D D'Andrea

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Citations

Dec 18, 2013·Stem Cells and Development·Déborah Afonso CornélioSilvia Regina Batistuzzo de Medeiros
Jan 16, 2013·Oncogene·M T Hayashi, J Karlseder
Jan 17, 2015·Journal of the American College of Cardiology·Nirmala HariharanMark A Sussman
Jan 14, 2016·Breast Cancer Research : BCR·Purificación FeijooAnna Genescà
Oct 18, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·A M MonganJ O'Sullivan
Feb 10, 2016·International Journal of Cancer. Journal International Du Cancer·Aurélie ErnstPeter Lichter
Mar 19, 2015·Environmental and Molecular Mutagenesis·Antonella RussoFrancesca Degrassi
Jul 18, 2014·Revista brasileira de hematologia e hemoterapia·Tamara BorgonovoPaulo Roberto Slud Brofman
Nov 20, 2012·Cell Cycle·Larissa LordierYunhua Chang
Jun 1, 2016·The Journal of Cell Biology·Esther AixIgnacio Flores
Sep 6, 2012·Current Opinion in Cell Biology·Anthony J Cesare, Jan Karlseder
Apr 4, 2017·Experimental Cell Research·Chenhui HuangWeihang Chai
Jun 14, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bogdan I Gerashchenko
Jan 20, 2018·International Journal of Molecular Sciences·Aina Bernal, Laura Tusell
Feb 8, 2018·Chromosoma·Nicolaas C Baudoin, Daniela Cimini
Jul 19, 2018·International Journal of Molecular Sciences·Aina BernalLaura Tusell
Jan 4, 2018·Nature Reviews. Clinical Oncology·Laurent SansregretCharles Swanton
Jun 14, 2020·Genes·Therese WilhelmValeria Naim
Jul 30, 2020·Cancers·Lena In der StrothAndré Lechel
Mar 13, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Alex GeorgeRadha Saraswathy
Jul 1, 2014·Molecular & Cellular Oncology·Stéphane Marcand
Oct 11, 2017·Genes & Genetic Systems·Makoto T Hayashi
Sep 18, 2018·Oncoimmunology·Fernando ArandaLaura Senovilla
Dec 14, 2018·F1000Research·Yinan Liu, Douglas Robinson
Dec 14, 2018·Genes·María Fernández-Casañas, Kok-Lung Chan
Jul 16, 2019·Cellular and Molecular Life Sciences : CMLS·Eleni Petsalaki, George Zachos
Oct 28, 2020·Life Science Alliance·Katsushi KagayaMakoto T Hayashi
Dec 12, 2012·Development·Donald T Fox, Robert J Duronio
Jun 20, 2021·Science Advances·Qian MeiSusan M Rosenberg
Oct 16, 2021·Frontiers in Cell and Developmental Biology·Marina Rodriguez-MuñozTeresa Anglada
Jul 15, 2015·Genes & Development·Chuan-Wei JangTerry Magnuson

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Methods Mentioned

BETA
flow cytometry
flow-cytometry
FACS
biopsies
PCR

Software Mentioned

Leica
Adobe Photoshop
ImageJ
Adobe After Effects
BDFacsDiva

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