PMID: 2498842May 11, 1989Paper

Prohead RNA of bacteriophage phi 29: size, stoichiometry and biological activity

Nucleic Acids Research
J WichitwechkarnD L Anderson

Abstract

We previously demonstrated (Guo et al., 1987. Nucl. Acids Res. 15, 7081-7090) that purified proheads of bacteriophage phi 29 contain an RNA of 120 bases which is essential for DNA packaging. Here we report that this RNA exists primarily as a polymer of ca. 174 residues in phage-infected cells and that ca. 54 bases are cleaved from its 3'-terminus by adventitious nucleases during the purification of proheads. The long and short forms of the RNA had similar activity in in vitro DNA packaging and phage assembly. We report the sequence of the long form of the RNA and show that similar long and short forms can be isolated from the proheads of the phi 29 relatives phi 21, phi 15 and SF5. The concentration dependence in the reconstitution of RNA-free proheads suggests that one copy of the RNA is sufficient to restore DNA packaging activity to RNA-free proheads. However, quantitative measurements indicate that 5 to 6 copies of the RNA are present on proheads isolated from phage-infected cells.

References

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Citations

Nov 30, 2002·Biochimie·Nancy Bourassa, François Major
Aug 11, 2012·Nucleic Acids Research·Elena HarjesHiroshi Matsuo
Jan 27, 2016·Wiley Interdisciplinary Reviews. RNA·Alyssa C HillSusan J Schroeder
Sep 10, 2008·Journal of Molecular Biology·Wei ZhaoShelley Grimes
Aug 5, 2008·Journal of Molecular Biology·Jaya S KotiDwight L Anderson
Mar 1, 2012·Wiley Interdisciplinary Reviews. RNA·Xin YeDecheng Yang
Feb 20, 1992·Journal of Molecular Biology·J WichitwechkarnD Anderson
Jul 1, 1990·Journal of Structural Biology·D Anderson, J W Bodley
Oct 20, 1990·Journal of Molecular Biology·S Grimes, D Anderson
Nov 14, 2006·Structure·Kyung H ChoiMichael G Rossmann
Mar 14, 1997·Journal of Molecular Biology·S Grimes, D Anderson
Oct 2, 2015·Journal of Virology·Wei ZhaoShelley Grimes
Jun 5, 2003·Nature Structural Biology·Marc C MoraisMichael G Rossmann
Feb 28, 1998·The Journal of Biological Chemistry·F Zhang, D Anderson
Aug 10, 2019·Nucleic Acids Research·Rujie CaiAilong Ke
Apr 1, 1993·Journal of Bacteriology·B S RajagopalD L Anderson
Jan 1, 1997·Journal of Virology·M Trottier, P Guo
Nov 2, 2021·The Journal of Physical Chemistry. B·Boyang ZhuoJingyuan Li

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