Oct 30, 2013

Prohibitin is required for transcriptional repression by the WT1-BASP1 complex

Oncogene
Eneda ToskaStefan Ge Roberts

Abstract

The Wilms' tumor-1 protein (WT1) is a transcriptional regulator that can either activate or repress genes controlling cell growth, apoptosis and differentiation. The transcriptional corepressor BASP1 interacts with WT1 and mediates WT1's transcriptional repression activity. BASP1 is contained within large complexes, suggesting that it works in concert with other factors. Here we report that the transcriptional repressor prohibitin is part of the WT1-BASP1 transcriptional repression complex. Prohibitin interacts with BASP1, colocalizes with BASP1 in the nucleus, and is recruited to the promoter region of WT1 target genes to elicit BASP1-dependent transcriptional repression. We demonstrate that prohibitin and BASP1 cooperate to recruit the chromatin remodeling factor BRG1 to WT1-responsive promoters and that this results in the dissociation of CBP from the promoter region of WT1 target genes. As seen with BASP1, prohibitin can associate with phospholipids. We demonstrate that the recruitment of PIP2 and HDAC1 to WT1 target genes is also dependent on the concerted activity of BASP1 and prohibitin. Our findings provide new insights into the function of prohibitin in transcriptional regulation and uncover a BASP1-prohibitin complex ...Continue Reading

Mentioned in this Paper

SMARCA4 wt Allele
Gene Expression Regulation, Neoplastic
Transcriptional Regulation
Transcription Repressor/Corepressor
SMARCA4 gene
HDAC1 gene
SMARCA4 protein, human
Complex (molecular entity)
Transcription, Genetic
Phb-1 protein, C elegans

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