Proinflammatory Activity of Primarily Infected Endodontic Content against Macrophages after Different Phases of the Root Canal Therapy

Journal of Endodontics
Ariane Cassia Salustiano MarinhoBrenda P F A Gomes

Abstract

This study investigated the presence of target bacterial species and the levels of endotoxins in teeth with apical periodontitis. Levels of inflammatory mediators (interleukin [IL]-1β and tumor necrosis factor [TNF]-α) were determined after macrophage stimulation with endodontic content after different phases of endodontic therapy using different irrigants. Thirty primarily infected root canals were randomly assigned into 3 groups according to the irrigant used for root canal preparation (n = 10 per group): GI: 2.5% sodium hypochlorite, GII: 2% chlorhexidine gel, and GIII (control group): saline solution. Root canal samples were taken by using paper points before (s1) and after root canal instrumentation (s2), subsequently to 17% EDTA (s3), after 30 days of intracanal medication (Ca[OH]2 + saline solution) (s4), and before root canal obturation (s5). Polymerase chain reaction (16S recombinant DNA) and limulus amebocyte lysate assay were used for bacterial and endotoxin detection, respectively. Macrophages were stimulated with the root canal contents for IL-1β/TNF-α measurement using enzyme-linked immunosorbent assay. Porphyromonas gingivalis (17/30), Porphyromonas endodontalis (15/30), and Prevotella nigrescens (11/30) were the...Continue Reading

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Citations

Apr 18, 2018·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Monique Costa Moreira FrançaCarlos Henrique Ribeiro Camargo
Sep 22, 2017·European Journal of Dentistry·Frederico Canato MartinhoGustavo Giacomelli Nascimento
May 24, 2020·Clinical Oral Investigations·Pablo VelosoMarcela Hernández
Oct 27, 2018·Brazilian Oral Research·Brenda Paula Figueiredo de Almeida Gomes, Daniel Rodrigo Herrera
Nov 24, 2019·Archives of Oral Biology·Nubia Rosa PriettoFábio Renato Manzolli Leite
Jan 23, 2021·International Endodontic Journal·C LoureiroR C Jacinto

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