Proinflammatory changes in human umbilical cord vein endothelial cells can be induced neither by native nor by modified CRP

International Immunology
Melinda OroszlánLászló Cervenak

Abstract

The role of C-reactive protein (CRP) in atherosclerosis is controversial. It is not clear, either, if the presumed endothelium-activating effect of CRP resides in native CRP (nCRP) or in a conformational isoform of CRP known as modified CRP (mCRP). In the present study we evaluated and compared the effect of nCRP, recombinant modified CRP (rmCRP) and urea-modified CRP (umCRP) on human umbilical vein endothelial cells (HUVECs). CRP preparations were carefully analyzed by biochemical, immunological and cell biological methods in order to avoid endotoxin or sodium azide contamination as well as inappropriate conformational changes, which together had possibly been the main reason for the previously published controversial results. Neither nCRP nor mCRP showed significant cytotoxicity up to 100 microg ml(-1) at 24 h but high concentrations of CRPs induced cell death at 48 h. rmCRP but not nCRP nor umCRP showed membrane binding to HUVEC by confocal microscopy. However, none of the CRP forms induced intercellular cell adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin expression or IL-8 production. Monocyte chemotactic protein-1 production was weakly inhibited by high concentration of both nCRP and rmCRP, analyzed by ...Continue Reading

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Citations

May 6, 2009·Seminars in Immunopathology·Paolo CalabròEdward T H Yeh
Jun 13, 2009·Journal of Receptor and Signal Transduction Research·Eszter HerczenikLászló Cervenak
Jun 22, 2010·Drug Delivery·Praveen K DubeyS P Vyas
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Feb 4, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Veronika MakóLászló Cervenak
May 28, 2019·Frontiers in Immunology·Márta L DebreczeniLászló Cervenak
Aug 12, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Márton MegyeriPéter Gál
Feb 26, 2020·Scientific Reports·Márta Lídia DebreczeniRobert Horvath
Nov 10, 2018·ACS Applied Materials & Interfaces·Qiqin WangZhengjin Jiang

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