Proinflammatory effects of pancreatic elastase are mediated through TLR4 and NF-kappaB

Biochemical and Biophysical Research Communications
A J HietarantaEsko Kemppainen

Abstract

Pancreatic elastase has been implicated in the pathophysiology of severe acute pancreatitis, characterized by systemic inflammatory response, distant organ failure, and high mortality. Here we show that pancreatic elastase activates transcription factors NF-kappaB, AP-1, and NFAT in human myeloid cells (U-937 and THP-1) in culture. Pancreatic elastase also induces TNF-alpha secretion and increased expression of CD11b in THP-1 cells which can be inhibited by neutralizing anti-Toll-like receptor 4 (TLR4) antibodies. NF-kappaB blocking agents (MG-132, PGA1) prevented elastase-induced TNF-alpha secretion from THP-1 cells. Our results suggest that pancreatic elastase-induced proinflammatory effects are mediated by TLR4 and NF-kappaB in human myeloid cells.

References

May 2, 1997·The Journal of Biological Chemistry·P R YoungJ C Lee
Oct 12, 2000·Molecular and Cellular Endocrinology·T IshikawaT Fujita
Feb 13, 2001·Biochemical and Biophysical Research Communications·A J HietarantaM L Steer

❮ Previous
Next ❯

Citations

May 13, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Jun-Li DingZong-Guang Zhou
May 6, 2011·The Journal of Biological Chemistry·Gabriela Panter, Roman Jerala
Jul 16, 2008·The Journal of Biological Chemistry·Prasad RallabhandiStefanie N Vogel
Jul 29, 2010·Pancreas·Xiping ZhangXinge Jiang
Jul 30, 2009·Journal of Gastroenterology and Hepatology·Adam G Testro, Kumar Visvanathan
Oct 14, 2009·Journal of Gastroenterology and Hepatology·Minoti ApteJeremy Wilson
May 29, 2008·World Journal of Gastroenterology : WJG·Xi ChenJi-Dong Liu
Jun 24, 2010·World Journal of Gastroenterology : WJG·Xian-Ming XiaHeng Lu
May 9, 2012·World Journal of Gastroenterology : WJG·Xian-Ming XiaHai-Ling Ruan
Feb 23, 2013·World Journal of Gastroenterology : WJG·Juan VazRoland Andersson
May 18, 2011·World Journal of Gastrointestinal Pharmacology and Therapeutics·Sabrina Gea-Sorlí, Daniel Closa
Mar 4, 2011·Cellular and Molecular Life Sciences : CMLS·Alessandra A FilardyGeorge A DosReis
Mar 18, 2008·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zongfang LiRui Zhou
Oct 11, 2005·Free Radical Biology & Medicine·Tamás LetohaBotond Penke
Jul 11, 2007·Journal of Endotoxin Research·Min-Fu Tsan, Baochong Gao
Jul 6, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Gautam BandyopadhyayGloria S Pryhuber
Feb 12, 2010·Expert Opinion on Therapeutic Targets·Pedro Pimentel-NunesAdelino F Leite-Moreira
Jul 5, 2011·Molecular Biology Reports·Lu ZhongkaiChen Weichang
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fabian GeislerRoland M Schmid
Apr 25, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Laura GamanMarilena Gilca
Mar 28, 2008·Scandinavian Journal of Gastroenterology·Jakob AxelssonRoland Andersson
Apr 10, 2019·American Journal of Respiratory Cell and Molecular Biology·Shigen HayashiNobuyuki Hizawa
Sep 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Flavia L Ribeiro-GomesGeorge A DosReis
May 20, 2017·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Catherine A FossMartin G Pomper

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.