Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells

International Journal of Molecular Medicine
Li MaShang-Long Yao

Abstract

S100A8/A9, a heterodimer of the two calcium-binding proteins S100A8 and S100A9, has emerged as an important proinflammatory mediator in acute and chronic inflammation. However, whether S100A8/A9 is implicated in microglial‑induced neuroinflammatory response remains unclear. Here, we found that S100A8/A9 significantly increased the secretion of proinflammatory cytokines inclu-ding tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in cultured BV-2 microglial cells. Inhibition of the Toll-like receptor 4 (TLR4) and the receptor for advanced glycation end-products (RAGE) with C225 and a RAGE-blocking antibody, respectively significantly reduced the secretion of TNF-α and IL-6 from S100A8/A9-stimulated BV-2 microglial cells. Furthermore, S100A8/A9 markedly enhanced the nuclear translocation of NF-κB p65 and the DNA-binding activities of NF-κB in BV-2 microglial cells, and suppression of ERK and JNK/MAPK signaling pathways by PD98059 or SP600125 significantly inhibited NF-κB activity and the release of TNF-α and IL-6 in the S100A8/A9-treated BV-2 microglial cells. Our data also showed that inhibition of NF-κB with pyrrolidine dithiocarbamate (PDTC) significantly reduced the secretion of TNF-α and IL-6 from BV-2 microglial cell...Continue Reading

References

Jun 1, 1997·Trends in Neurosciences·L A O'Neill, C Kaltschmidt
Jul 6, 2000·The EMBO Journal·A O AliprantisA Zychlinsky
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carle RyckmanPhilippe A Tessier
Mar 20, 2003·Microscopy Research and Technique·Rosario Donato
Mar 20, 2003·Microscopy Research and Technique·Wolfgang NackenClaus Kerkhoff
Apr 17, 2003·Trends in Immunology·Johannes RothCord Sunderkötter
Jun 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Seija LehnardtTimothy Vartanian
Nov 17, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Katalin KarikóFrank A Welsh
Sep 1, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Angelika BierhausPeter P Nawroth
Sep 28, 2005·Neurochemical Research·Mark P Mattson
Oct 29, 2005·Neurobiology of Aging·C E ShepherdG M Halliday
Mar 17, 2006·Journal of Neuroscience Research·Tammy Kielian
Oct 3, 2006·Journal of Neuroimmunology·Bibhuti B MishraJudy M Teale
Jul 4, 2007·The Journal of Cell Biology·Anthony P MandersonJennifer L Stow
Dec 15, 2007·The Journal of Clinical Investigation·Evis HarjaAnn Marie Schmidt
Jan 16, 2008·Sub-cellular Biochemistry·C W HeizmannA Galichet
May 20, 2008·Neurobiology of Disease·Ulkan KilicDirk M Hermann
Sep 16, 2008·Neuroscience·B W McCollN J Rothwell
Sep 24, 2008·Neuroscience·B J MarshM P Stenzel-Poore
Oct 20, 2009·Biochimica Et Biophysica Acta·Gina ZieglerGeorge Trendelenburg
Apr 8, 2011·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·K EggersV Stangl
Jun 12, 2012·Biochemical and Biophysical Research Communications·Young LeeJeung-Hoon Lee
Nov 28, 2012·Autoimmunity Reviews·Maria Sole ChimentiRoberto Perricone
May 28, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Haixia HuLidian Chen
Aug 30, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jin ZhuShu Zhang

❮ Previous
Next ❯

Citations

Aug 8, 2018·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Fatemeh ShabaniNematollah Gheibi
Dec 29, 2018·Journal of Clinical Periodontology·Sandeep KarnaHyun-Duck Kim
Feb 6, 2019·Molecular Carcinogenesis·I Wayan SumardikaMasakiyo Sakaguchi
Dec 31, 2019·Cell Biochemistry and Function·Miloš DiklićVladan P Čokić
Jul 18, 2020·Molecular Neurodegeneration·Tiantian GuoYingjun Zhao
Jun 27, 2018·Frontiers in Immunology·Siwen WangJian Ma
May 26, 2020·Italian Journal of Pediatrics·Yuanyuan ZhuBin Sun
Dec 19, 2020·Frontiers in Microbiology·Tanveer AhmadSyed Mansoor Ali
Dec 4, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xuan XiaoChi Zhang
Dec 11, 2019·International Journal of Biological Macromolecules·Alexey S KazakovSergei E Permyakov
Dec 31, 2019·Biochemistry·Aaron T Bozzi, Elizabeth M Nolan
Feb 4, 2021·International Journal of Molecular Sciences·Ji Sun HaSeung-Ju Yang
Apr 4, 2020·Microbial Pathogenesis·Shalee K CarlsonEric Wilson
Feb 23, 2021·Frontiers in Neuroscience·Guo-Yuan HeXiao-Chun Jiang
Apr 4, 2021·International Journal of Molecular Sciences·Mehdi EshraghiSaeid Ghavami
Jun 20, 2021·Gut·Almina JukicTimon E Adolph
Apr 6, 2018·ACS Chemical Neuroscience·Igor A IashchishynLudmilla A Morozova-Roche

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA
electrophoresis
nuclear translocation

Software Mentioned

SPSS
Quantity One

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.