Proinflammatory Response of Human Trophoblastic Cells to Brucella abortus Infection and upon Interactions with Infected Phagocytes

Biology of Reproduction
Andrea G FernándezPablo C Baldi

Abstract

Trophoblasts are targets of infection by Brucella spp. but their role in the pathophysiology of pregnancy complications of brucellosis is unknown. Here we show that Brucella abortus invades and replicates in the human trophoblastic cell line Swan-71 and that the intracellular survival of the bacterium depends on a functional virB operon. The infection elicited significant increments of interleukin 8 (IL8), monocyte chemotactic protein 1 (MCP-1), and IL6 secretion, but levels of IL1beta and tumor necrosis factor-alpha (TNF-alpha) did not vary significantly. Such proinflammatory response was not modified by the absence of the Brucella TIR domain-containing proteins BtpA and BtpB. The stimulation of Swan-71 cells with conditioned medium (CM) from B. abortus-infected human monocytes (THP-1 cells) or macrophages induced a significant increase of IL8, MCP-1 and IL6 as compared to stimulation with CM from non-infected cells. Similar results were obtained when stimulation was performed with CM from infected neutrophils. Neutralization studies showed that IL1beta and/or TNF-alpha mediated the stimulating effects of CM from infected phagocytes. Reciprocally, stimulation of monocytes and neutrophils with CM from Brucella-infected trophobl...Continue Reading

References

Jul 1, 1974·American Journal of Obstetrics and Gynecology·M SarramP Gazanfarpour
Jan 1, 1996·Comparative Immunology, Microbiology and Infectious Diseases·L E Samartino, F M Enright
Oct 6, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·R J BildfellN Smart
Apr 3, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M Y KhanZ A Memish
Dec 26, 2001·Journal of the Society for Gynecologic Investigation·G GriesingerM Knöfler
Nov 25, 2003·American Journal of Reproductive Immunology : AJRI·Morgan R PeltierMary B Brown
Jun 3, 2005·The New England Journal of Medicine·Georgios PappasEpameinondas Tsianos
Dec 21, 2005·Research in Microbiology·Jean Celli
Jan 28, 2006·The Lancet Infectious Diseases·Georgios PappasEpameinondas V Tsianos
Sep 12, 2008·Current Protocols in Stem Cell Biology·Anita Laitinen, Jarmo Laine
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eugenia de la TorreVikki M Abrahams
Apr 21, 2009·Microbes and Infection·Astrid ZwerdlingGuillermo H Giambartolomei
Sep 8, 2009·The Veterinary Journal·Alcina V Carvalho NetaRenato L Santos
Jan 13, 2010·Reproductive Sciences·Takeshi Nagamatsu, Danny J Schust
May 11, 2010·Journal of Hepatology·M Victoria DelpinoPablo C Baldi
Jul 14, 2010·Molecular Oral Microbiology·S D RieweR J Lamont
Jun 17, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ingrid CardenasVikki M Abrahams
Jan 11, 2013·The Journal of Infectious Diseases·Suzana Pinto SalcedoJean-Pierre Gorvel
Jul 11, 2013·Revue Scientifique Et Technique·F P PoesterR L Santos
Jul 13, 2013·Frontiers in Cellular and Infection Microbiology·Suzana P SalcedoJean-Pierre Gorvel

❮ Previous
Next ❯

Citations

Nov 27, 2018·FEMS Microbiology Letters·Rebeca Singh Sidhu-MuñozNieves Vizcaíno
Oct 17, 2017·PloS One·Andrea G FernándezPablo C Baldi
Dec 12, 2017·Current Tropical Medicine Reports·Angela M Arenas-GamboaThomas A Ficht
Nov 18, 2020·Pathogens·Magalí G BialerAngeles Zorreguieta
Feb 12, 2021·Microbiology and Molecular Biology Reviews : MMBR·R Martin RoopDaniel W Martin
May 15, 2021·Frontiers in Cellular and Infection Microbiology·Magalí G BialerAngeles Zorreguieta
Oct 16, 2020·Microbiology and Molecular Biology Reviews : MMBR·Edgardo Moreno, Elías Barquero-Calvo
Aug 3, 2021·Frontiers in Microbiology·Lauren W Stranahan, Angela M Arenas-Gamboa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.