Proinflammatory Role of Angiotensin II in the Aorta of Normotensive Mice

BioMed Research International
Rariane Silva de LimaSilvia Lacchini

Abstract

Angiotensin II plays important functions in cardiovascular system mediating actions leading to inflammatory responses such as activation of VSMC in order to produce ROS, inflammatory cytokines, chemokines, and adhesion molecules. Changes in angiotensin II production could stimulate the recruitment and activation of myeloid cells initiating local inflammatory response without effect on BP. We aimed to verify if angiotensin II induces an inflammatory response in the aorta and if it correlates with variations in BP. C57Bl/6 mice treated with saline solution (0.9%, control group) or angiotensin II (30ng/kg, Ang II group) were used. BP and HR levels were measured. Immunohistochemistry for IL1-β, TGF-β, iNOS, CD45, and α-actin was performed in the aorta. BP and HR do not change. A biphasic response was observed both for IL1-β and TGF-β expression and also for the presence of CD45 positive cells, with an acute increase (between 30 and 60 minutes) and a second increase, between 24 and 48 hours. Positive staining for iNOS increased in the earlier period (30 minutes) in perivascular adipose tissue and in a longer period (48 hours) in tunica adventitia. Immunoblotting to α-actin showed no alterations, suggesting that the applied dose of a...Continue Reading

References

Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ernesto L Schiffrin, Rhian M Touyz
Apr 8, 2006·Physiological Reviews·Alain Tedgui, Ziad Mallat
Sep 19, 2007·The Journal of Experimental Medicine·Tomasz J GuzikDavid G Harrison
Jun 27, 2008·Trends in Pharmacological Sciences·Chiara MarchesiErnesto L Schiffrin
Oct 23, 2010·Nature Reviews. Cancer·Amee J GeorgeRoss D Hannan
Jan 26, 2011·Hypertension·Tlili BarhoumiErnesto L Schiffrin
May 8, 2014·International Journal of Inflammation·Maricica PacurariKenneth Ndebele
Jun 14, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Jeffrey P MooreGrant R Drummond
Oct 6, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Salema Jafri, Mark L Ormiston
Nov 25, 2017·Translational Research : the Journal of Laboratory and Clinical Medicine·Sailesh C Harwani

❮ Previous
Next ❯

Software Mentioned

ImageJ
GraphPad
GraphPad Prism
Fiji

Related Concepts

Related Feeds

Biomarkers for Cardiovascular Risk Assessment

Sensitive and accurate biomarkers used in cardiovascular risk prediction can potentially be used to manage the risk of cardiovascular disease. Discover the latest research on Biomarkers for Cardiovascular Risk Assessment here. Discover the latest research on Biomarkers for Cardiovascular Risk Assessment here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.