Projection-Based 3D Printing of Cell Patterning Scaffolds with Multiscale Channels

ACS Applied Materials & Interfaces
Dai XueShaochen Chen

Abstract

To fully actualize artificial, cell-laden biological models in tissue engineering, such as 3D organoids and organs-on-a-chip systems, cells need to be patterned such that they can precisely mimic natural microenvironments in vitro. Despite increasing interest in this area, patterning cells at multiscale (∼10 μm to 10 mm) remains a significant challenge in bioengineering. Here, we report a projection-based 3D printing system that achieves rapid and high-resolution fabrication of hydrogel scaffolds featuring intricate channels for multiscale cell patterning. Using this system, we were able to use biocompatible poly(ethylene glycol)diacrylate in fabricating a variety of scaffold architectures, ranging from regular geometries such as serpentine, spiral, and fractal-like to more irregular/intricate geometries, such as biomimetic arborescent and capillary networks. A red food dye solution was able to freely fill all channels in the scaffolds, from the trunk (>1100 μm in width) to the small branch (∼17 μm in width) without an external pump. The dimensions of the printed scaffolds remained stable over 3 days while being immersed in Dulbecco's phosphate-buffered saline at 37 °C, and a penetration analysis revealed that these scaffolds a...Continue Reading

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Citations

Sep 25, 2019·Faraday Discussions·Andrea S CarliniNathan C Gianneschi
Mar 4, 2020·Advanced Healthcare Materials·Jing NieYong He
Jun 9, 2020·Small·Giuseppe ArrabitoBruno Pignataro
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Mar 13, 2021·Advanced Functional Materials·Sharon FleischerGordana Vunjak-Novakovic
Mar 20, 2021·ACS Applied Materials & Interfaces·Daniel VeraElena Martinez
Jan 7, 2021·ACS Applied Materials & Interfaces·Yue WangLijie Grace Zhang
Sep 2, 2020·Chemical Reviews·Ryan W BarrsYing Mei
Mar 27, 2019·ACS Applied Materials & Interfaces·Tingting ZhaoWei Huang

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