Abstract
In this study we examined the microbial community composition and operating metabolisms on the surface and in the permafrost of Deception Island, (Antarctica) with an on site antibody microarray biosensor. Samples (down to a depth of 4.2 m) were analysed with LDChip300 (Life Detector Chip), an immunosensor containing more than 300 antibodies targeted to bacterial and archaeal antigens. The immunograms showed positive antigen-antibody reactions in all surface samples (lichens, pyroclasts) and the top layer of the permafrost. The results indicated the presence of exopolysaccharides, bacteria belonging to the Alpha-, Delta- and Gammaproteobacteria, Bacteroidetes, Gram-positive Actinobacteria and Firmicutes, as well as archaeal species, most probably Methanobacterium spp. Positive reactions with antibodies to proteins and peptides revealed the presence of nitrogen fixation (NifHD, GlnB, HscA), methanogenic (McrB), iron homeostasis and iron scavenging (ferritins and DPS proteins) proteins, as well as ABC transporters, which indicated that these processes were operating at the time of sampling. These results were validated with other molecular ecology techniques such as oligonucleotide microarrays, 16S bacterial rRNA gene sequence an...Continue Reading
References
Apr 1, 1997·International Journal of Systematic Bacteriology·K SuzukiK Komagata
Jul 1, 1997·International Journal of Systematic Bacteriology·J P BowmanT A McMeekin
Oct 23, 1997·International Journal of Systematic Bacteriology·J P BowmanT A McMeekin
Nov 11, 1999·International Journal of Systematic Bacteriology·C KnoblauchB B Jørgensen
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Aug 13, 2004·Environmental Microbiology·R RabusH-P Klenk
Dec 23, 2004·Systematic and Applied Microbiology·Peter HirschRobert Anderson
Feb 16, 2005·Astrobiology·Mary Higby SchweitzerSeth Pincus
Jun 11, 2005·Environmental Microbiology·Mirko LunauMeinhard Simon
Mar 22, 2006·Extremophiles : Life Under Extreme Conditions·Blaire StevenLyle G Whyte
Aug 4, 2006·Applied and Environmental Microbiology·W Matthew Sattley, Michael T Madigan
Mar 3, 2007·International Journal of Systematic and Evolutionary Microbiology·Maria Sizova, Nicolai Panikov
May 8, 2007·Astrobiology·D A GilichinskyJ M Tiedje
Jul 20, 2007·Environmental Microbiology·Bin LinWilfred F M Röling
Aug 29, 2007·Astrobiology·John ParnellJorge Vago
Feb 6, 2008·Environmental Microbiology·Patricia GarridoVíctor Parro
Oct 8, 2008·Analytical Chemistry·Luis A RivasVíctor Parro
Dec 25, 2008·Astrobiology·Víctor ParroCarol R Stoker
Feb 5, 2009·Canadian Journal of Microbiology·Joseph G MarxJody W Deming
Jul 4, 2009·Science·P H SmithA P Zent
Sep 22, 2009·Applied and Environmental Microbiology·Satoshi IshiiKeishi Senoo
Apr 16, 2010·The ISME Journal·Etienne YergeauCharles W Greer
Feb 8, 2011·Astrobiology·Víctor ParroJavier Gómez-Elvira
Feb 8, 2011·Astrobiology·Victor ParroJavier Gómez-Elvira
Feb 10, 2011·Standards in Genomic Sciences·Johannes SikorskiAlla Lapidus
Feb 12, 2011·Extremophiles : Life Under Extreme Conditions·Kevin B HallbergD Barrie Johnson
Mar 16, 2011·Environmental Microbiology·Luis A RivasVíctor Parro
Dec 14, 2011·Astrobiology·Victor ParroJavier Gómez-Elvira
Citations
May 8, 2013·Environmental Microbiology·Kateřina JanatkováMartin Kopecký
Nov 19, 2013·Geobiology·F Puente-SánchezV Parro
Dec 23, 2014·PloS One·Yolanda BlancoVíctor Parro
Jan 8, 2015·Environmental Science & Technology·Yolanda BlancoVictor Parro
Nov 29, 2016·Astrobiology·Víctor ParroNathalie A Cabrol
Apr 12, 2018·Astrobiology·Mercedes Moreno-PazVictor Parro
Nov 1, 2017·Molecular Ecology·Yong-Liang ChenYuan-He Yang
Oct 11, 2017·Astrobiology·Yolanda BlancoVíctor Parro
May 13, 2014·Nature Reviews. Microbiology·Janet K Jansson, Neslihan Taş
Jul 25, 2019·Astrobiology·María Ángeles LezcanoVíctor Parro
Jan 25, 2020·Astrobiology·Catherine MaggioriLyle Whyte
Jan 10, 2020·Astrobiology·Laura Sánchez-GarcíaVictor Parro
Mar 16, 2017·Microbial Ecology·Rosabruna La FerlaMauro Guglielmin
Aug 10, 2019·Frontiers in Microbiology·Miguel Ángel Fernández-MartínezVíctor Parro
Jan 31, 2019·Frontiers in Microbiology·Laura Sanchez-GarciaDaniel Carrizo
Jul 30, 2019·Frontiers in Microbiology·Malin BombergAnne Kontula
Feb 6, 2020·Frontiers in Microbiology·María Asunción Campanero-RhodesDolores Solís
Sep 11, 2018·The Science of the Total Environment·Yolanda BlancoFernando Puente-Sánchez
Jan 5, 2021·Frontiers in Microbiology·Ignacio Gallardo-CarreñoVíctor Parro
Mar 17, 2020·Microbial Pathogenesis·Pamela AfoudaDidier Raoult