Prokaryotic communities and operating metabolisms in the surface and the permafrost of Deception Island (Antarctica)

Environmental Microbiology
Yolanda BlancoVictor Parro

Abstract

In this study we examined the microbial community composition and operating metabolisms on the surface and in the permafrost of Deception Island, (Antarctica) with an on site antibody microarray biosensor. Samples (down to a depth of 4.2 m) were analysed with LDChip300 (Life Detector Chip), an immunosensor containing more than 300 antibodies targeted to bacterial and archaeal antigens. The immunograms showed positive antigen-antibody reactions in all surface samples (lichens, pyroclasts) and the top layer of the permafrost. The results indicated the presence of exopolysaccharides, bacteria belonging to the Alpha-, Delta- and Gammaproteobacteria, Bacteroidetes, Gram-positive Actinobacteria and Firmicutes, as well as archaeal species, most probably Methanobacterium spp. Positive reactions with antibodies to proteins and peptides revealed the presence of nitrogen fixation (NifHD, GlnB, HscA), methanogenic (McrB), iron homeostasis and iron scavenging (ferritins and DPS proteins) proteins, as well as ABC transporters, which indicated that these processes were operating at the time of sampling. These results were validated with other molecular ecology techniques such as oligonucleotide microarrays, 16S bacterial rRNA gene sequence an...Continue Reading

References

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Citations

May 8, 2013·Environmental Microbiology·Kateřina JanatkováMartin Kopecký
Jan 8, 2015·Environmental Science & Technology·Yolanda BlancoVictor Parro
May 13, 2014·Nature Reviews. Microbiology·Janet K Jansson, Neslihan Taş
Feb 6, 2020·Frontiers in Microbiology·María Asunción Campanero-RhodesDolores Solís
Mar 17, 2020·Microbial Pathogenesis·Pamela AfoudaDidier Raoult

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