Prokaryotic expression of a constitutively expressed Tephrosia villosa defensin and its potent antifungal activity

Applied Microbiology and Biotechnology
S VijayanP B Kirti

Abstract

Plant defensins are small, highly stable, cysteine-rich antimicrobial peptides produced by the plants for inhibiting a broad-spectrum of microbial pathogens. Some of the well-characterized plant defensins exhibit potent antifungal activity on certain pathogenic fungal species only. We characterized a defensin, TvD1 from a weedy leguminous herb, Tephrosia villosa. The open reading frame of the cDNA was 228 bp, which codes for a peptide with 75 amino acids. Expression analyses indicated that this defensin is expressed constitutively in T. villosa with leaf, stem, root, and seed showing almost similar levels of high expression. The recombinant peptide (rTvD1), expressed in the Escherichia coli expression system, exhibited potent in vitro antifungal activity against several filamentous soil-borne fungal pathogens. The purified peptide also showed significant inhibition of root elongation in Arabidopsis seedlings, subsequently affecting the extension of growing root hairs indicating that it has the potential to disturb the plant growth and development.

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Citations

May 19, 2011·Yi chuan = Hereditas·Lan-Bao FuWei-Hua Liu
Apr 1, 2014·Peptides·Nelson Marmiroli, Elena Maestri
May 12, 2009·Peptides·André de Oliveira Carvalho, Valdirene Moreira Gomes
Jul 7, 2009·Biotechnology and Applied Biochemistry·Yifeng Li
Jul 3, 2013·Experimental Parasitology·Géssika Silva SouzaAndré de Oliveira Carvalho
Apr 26, 2014·Frontiers in Microbiology·Ariane F LacerdaMaria F Grossi de Sa
Sep 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Thibaut TheryElke K Arendt

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