Prokaryotic Kdp-ATPase: recent insights into the structure and function of KdpB

Journal of Molecular Microbiology and Biotechnology
Melina HauptK Altendorf

Abstract

P-type ATPases are amongst the most abundant enzymes that are responsible for active transport of ions across biological membranes. Within the last 5 years a detailed picture of the structure and function of these transport ATPases has emerged. Here, we report on the recent progress in elucidating the molecular mechanism of a unique, prokaryotic member of P-type ATPases, the Kdp-ATPase. The review focuses on the catalytic parts of the central subunit, KdpB. The structure of the nucleotide-binding domain was solved by NMR spectroscopy at high resolution and a model of the nucleotide-binding mode was presented. The nucleotide turned out to be 'clipped' into the binding pocket by a pi-pi interaction to F377 on one side and a cation-pi interaction to K395 on the other. The 395KGXXD/E motif and thus the nucleotide-binding mode seems to be conserved in all P-type ATPases, except the heavy metal-transporting (class IB) ATPases. Hence, it can be concluded that KdpB is currently misgrouped as class IA. Mutational studies on two highly conserved residues (D583 and K586) in the transmembrane helix 5 of KdpB revealed that they are indispensable in coupling ATP hydrolysis to ion translocation. Based on these results, two possible pathways f...Continue Reading

References

Mar 1, 1976·The Journal of General Physiology·D B RhoadsW Epstein
Jan 1, 1979·Annual Review of Biochemistry·L de Meis, A L Vianna
Dec 5, 1995·Biochemistry·S Lutsenko, J H Kaplan
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Mar 7, 1998·Journal of Molecular Evolution·K B Axelsen, M G Palmgren
Aug 7, 1998·Biochimica Et Biophysica Acta·P L JorgensenP A Pedersen
Dec 30, 1998·Trends in Biochemical Sciences·L Aravind, E V Koonin
Mar 15, 2001·European Journal of Biochemistry·M Gassel, K Altendorf
Feb 7, 2002·Journal of Molecular Biology·Chen XuDavid L Stokes
Aug 9, 2002·Nature·Chikashi Toyoshima, Hiromi Nomura
Aug 27, 2002·Cytotherapy·S M SmithK van Besien
May 6, 2003·Nature Structural Biology·Mark HilgeJan Pieter Abrahams
Sep 19, 2003·Journal of Bioenergetics and Biomembranes·Gene A Scarborough
Sep 23, 2003·Journal of Molecular Biology·Kjell O Håkansson
Jun 12, 2004·Science·Thomas Lykke-Møller SørensenPoul Nissen
Jun 24, 2004·Journal of Biomolecular NMR·Melina HauptHorst Kessler
Jul 2, 2004·Nature·Chikashi Toyoshima, Tatsuaki Mizutani
Sep 24, 2004·Biochemistry·Marc Bramkamp, Karlheinz Altendorf
Jan 1, 1967·Annual Review of Biochemistry·R W Albers

❮ Previous
Next ❯

Citations

Jun 24, 2009·The Journal of Membrane Biology·Mark D Thever, Milton H Saier
Mar 6, 2010·Trends in Microbiology·Katharina Pflüger-Grau, Boris Görke
Jun 15, 2015·Biochemistry. Biokhimii︠a︡·D V DibrovaA Y Mulkidjanian
Dec 6, 2011·PLoS Biology·Barry J GrantRobert A Cross
Apr 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·James P StratfordMunehiro Asally
Oct 23, 2020·Antibiotics·Julia BespyatykhEgor Shitikov
Mar 11, 2008·Archives of Biochemistry and Biophysics·Francesco Tadini-BuoninsegniKlaus Fendler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.