Prolactin and MA-10 Leydig cell steroidogenesis: biphasic effects of prolactin and signal transduction

Endocrinology
E Weiss-MesserR J Barkey

Abstract

The present investigation was designed to study the direct role of PRL on testicular Leydig cell steroidogenesis, using the MA-10 murine Leydig tumor cell line as a model system. We have previously reported on the presence of specific PRL binding sites in those cells, and we now demonstrate the functionality of those sites and the biological responses induced by the binding of PRL. When cultured MA-10 cells were exposed for 24 h to increasing concentrations of PRL, washed, and then subjected to a 3-h human CG (hCG) stimulation test, a clear dose-dependent biphasic effect of PRL on the steroidogenic response was observed, even though PRL had no effect on MA-10 cell proliferation: at low PRL concentrations (0.1-10 ng/ml), hCG-induced steroidogenesis was stimulated (maximal stimulation by 1 ng/ml PRL being 200-250% of control); at higher concentrations, hCG-induced steroidogenesis was inhibited (60% inhibition was achieved by 1000 ng/ml PRL). When steroidogenesis was induced with various concentrations of cholera toxin, instead of hCG, no effect of the prior exposure to increasing concentrations of PRL was observed, indicating that PRL acts either at the level of the LH/hCG receptor or at some stage proximal to adenylate cyclase. ...Continue Reading

Citations

Mar 4, 2008·Molecular and Cellular Endocrinology·Chandrakesan Parthasarathy, Karundevi Balasubramanian
Aug 16, 2001·Critical Reviews in Toxicology·E J Calabrese
Jan 29, 2008·Advances in Therapy·Fulden SaraçAyşenur Memiş
Oct 4, 2007·American Journal of Physiology. Endocrinology and Metabolism·Valencia L WilliamsAmeae M Walker
Jun 6, 2018·Frontiers in Endocrinology·Zulvikar Syambani Ulhaq, Mitsuyo Kishida

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