Prolactin blocks the expression of receptor activator of nuclear factor κB ligand and reduces osteoclastogenesis and bone loss in murine inflammatory arthritis

Arthritis Research & Therapy
Maria G Ledesma-ColungaCarmen Clapp

Abstract

Prolactin (PRL) reduces joint inflammation, pannus formation, and bone destruction in rats with polyarticular adjuvant-induced arthritis (AIA). Here, we investigate the mechanism of PRL protection against bone loss in AIA and in monoarticular AIA (MAIA). Joint inflammation, trabecular bone loss, and osteoclastogenesis were evaluated in rats with AIA treated with PRL (via osmotic minipumps) and in mice with MAIA that were null (Prlr-/-) or not (Prlr+/+) for the PRL receptor. To help define target cells, synovial fibroblasts from Prlr+/+ mice were treated or not with proinflammatory cytokines ((Cyt), including TNFα, IL-1β, and interferon (IFN)γ) with or without PRL, and these synovial cells were co-cultured or not with bone marrow osteoclast progenitors from Prlr+/+ or Prlr-/- mice. In AIA, PRL treatment reduced joint swelling, increased trabecular bone area, lowered osteoclast density, and reduced mRNA levels of osteoclast-associated genes (tartrate-resistant acid phosphatase (Trap)), cathepsin K (Ctsk), matrix metalloproteinase 9 (Mmp9), and receptor activator of nuclear factor κB or RANK (Tnfrsf11a)), of genes encoding cytokines with osteoclastogenic activity (Tnfa, Il1b, Il6, and receptor activator of nuclear factor κB ligand...Continue Reading

References

Jul 29, 1999·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·M NeidhartS Gay
Feb 29, 2000·Arthritis and Rheumatism·E M GravalleseS R Goldring
Jun 17, 2000·The Journal of Endocrinology·A M CorbachoC Clapp
Aug 4, 2001·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·L C Hofbauer, A E Heufelder
Oct 16, 2002·Annals of the Rheumatic Diseases·D Holstead JonesJ M Penninger
Sep 4, 2003·The Journal of Biological Chemistry·Sunil SrivastavaNelson D Horseman
Sep 6, 2003·The Journal of Surgical Research·Reiner OberbeckMichael S Exton
Oct 19, 2004·Journal of Neuroscience Methods·Stephan D GauldieIain P Chessell
Apr 13, 2006·Journal of Cell Science·Yazmín MacotelaCarmen Clapp
May 2, 2006·The Journal of Endocrinology·C ZermeñoC Clapp
Nov 8, 2006·The Journal of Experimental Medicine·Kojiro SatoHiroshi Takayanagi
Apr 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Khandaker SiddiqueeJames Turkson
Sep 21, 2007·The New England Journal of Medicine·Steven M HollandBodo Grimbacher
Dec 7, 2007·Endocrine Reviews·Nira Ben-JonathanElizabeth W LaPensee
Sep 2, 2008·Arthritis and Rheumatism·Yaël A de ManJohanna M W Hazes
Feb 13, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Florence DeknuydtMaha Ayyoub
Mar 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kui Shin VooYong-Jun Liu
Feb 4, 2010·Seminars in Immunopathology·Erik Lubberts
Jun 19, 2010·Nature Reviews. Immunology·Shimon SakaguchiDavid A Hafler
Apr 12, 2011·Endocrine·Gherardo MazziottiAndrea Giustina
Aug 25, 2011·Arthritis Research & Therapy·Tobias Braun, Jochen Zwerina
Jun 20, 2012·Arthritis Research & Therapy·Maria J Martínez-CalatravaGabriel Herrero-Beaumont
Sep 26, 2012·Nature Reviews. Rheumatology·Georg Schett, Ellen Gravallese
Aug 3, 2013·The Journal of Clinical Investigation·Norma AdánCarmen Clapp
Aug 21, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Bibiana Moreno-CarranzaCarmen Clapp
Dec 24, 2013·Nature Medicine·Noriko KomatsuHiroshi Takayanagi

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Citations

Apr 29, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Georgina OrtizCarmen Clapp
Jun 24, 2017·Journal of Leukocyte Biology·Man Wai TangKris A Reedquist
Oct 26, 2018·Frontiers in Integrative Neuroscience·Gregory DussorArmen N Akopian
Aug 21, 2019·International Journal of Molecular Sciences·Wen-Ting LvChun-Jie Wang
Mar 5, 2021·Reviews in the Neurosciences·Edgar Ramos-MartinezMarco Cerbon
Mar 23, 2021·Frontiers in Endocrinology·Gabriela Ramirez-HernandezCarmen Clapp
Oct 29, 2020·Seminars in Cell & Developmental Biology·Ödül Karayazi AtıcıCarrie S Shemanko
Jun 12, 2021·Scientific Reports·Jila NasirzadeReinhard Gruber

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Methods Mentioned

BETA
ELISA
light microscopy
PCR

Software Mentioned

Pro Plus
Image
Sigma Stat
Quantity One

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