Prolactin decreases epidermal growth factor receptor kinase activity via a phosphorylation-dependent mechanism.

The Journal of Biological Chemistry
V J Quijano, L G Sheffield

Abstract

Previously, we have shown that prolactin inhibits epidermal growth factor (EGF)-induced mitogenesis in mouse mammary epithelial cells without altering the response to other growth promoting agents. This effect has been associated with reduced EGF-induced EGF receptor (EGFR) tyrosine phosphorylation, Grb-2 association, and Ras activation. Our current hypothesis is that prolactin induces an alteration in EGFR kinase activity via a phosphorylation-dependent mechanism. To test this hypothesis, we treated normal murine mammary gland cells with or without 100 ng/ml prolactin. EGFR isolated by wheat germ agglutinin affinity chromatography from nontreated cells exhibited substantial ligand-induced phosphorylation, and EGFR isolated from prolactin-treated cells displayed minimal EGF-induced EGFR phosphorylation, as well as decreased kinase activity toward exogenous substrates. The observed decrease in ligand-induced EGFR phosphorylation could not be attributed to either differential amounts of EGFR, decreased EGF binding affinity, or the presence of a phosphotyrosine phosphatase or ATPase. EGFR isolated from prolactin-treated cells exhibited increased phosphorylation on threonine. Removal of this phosphorylation with alkaline phosphatas...Continue Reading

References

Dec 1, 1978·Molecular and Cellular Endocrinology·S SakaiM R Banerjee
Sep 4, 1992·Science·S L Pelech, J S Sanghera
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K TakishimaM R Rosner
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Mar 1, 1989·Environmental Health Perspectives·F Borellini, T Oka
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M HoneggerJ Schlessinger
Dec 1, 1986·The Journal of Cell Biology·J Schlessinger
Oct 31, 1988·Biochemical and Biophysical Research Communications·R J BarkeyM L Dufau
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A R BuckleyD H Russell
Dec 15, 1988·Biochemical and Biophysical Research Communications·K TakishimaM R Rosner
Aug 1, 1994·Molecular and Cellular Biology·A G BatzerJ Schlessinger
May 1, 1995·Cell Biology International·J BoonstraP van Bergen en Henegouwen
Jan 1, 1994·Breast Cancer Research and Treatment·T Rajkumar, W J Gullick
Oct 1, 1995·Endocrine Reviews·R B Dickson, M E Lippman

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Citations

Dec 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·Manuel Rieber, Mary Strasberg Rieber
Feb 1, 2008·Journal of Mammary Gland Biology and Neoplasia·Stuart J Frank
Jan 26, 2008·Journal of Mammary Gland Biology and Neoplasia·Lisa M Arendt, Linda A Schuler
Mar 14, 2000·Archives of Medical Research·F LarreaA Ulloa-Aguirre
Apr 28, 2001·Molecular and Cellular Endocrinology·M D SchroederL A Schuler
Feb 15, 2002·Biological Chemistry·P O HackelA Ullrich
Mar 28, 2006·The American Journal of Pathology·Lisa M ArendtLinda A Schuler
Dec 10, 1999·The Journal of Biological Chemistry·S O KimS J Frank
Mar 6, 2004·Journal of Animal Science·L G Sheffield, J J Gavinski

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