Prolactin decreases expression of Runx2, osteoprotegerin, and RANKL in primary osteoblasts derived from tibiae of adult female rats

Canadian Journal of Physiology and Pharmacology
Narattaphol CharoenphandhuNateetip Krishnamra

Abstract

Hyperprolactinemia caused by physiological or pathological conditions, such as those occurring during lactation and prolactinoma, respectively, results in progressive osteopenia. The underlying mechanisms, however, are controversial. Prolactin (PRL) may directly attenuate the functions of osteoblasts, since these bone cells express PRL receptors. The present study therefore aimed to investigate the effects of PRL on the expression of genes related to the osteoblast functions by using quantitative real-time PCR technique. Herein, we used primary osteoblasts that were derived from the tibiae of adult rats and displayed characteristics of differentiated osteoblasts, including in vitro mineralization. Osteoblasts exposed for 48 h to 1000 ng/mL PRL, but not to 10 or 100 ng/mL PRL, showed decreases in the mRNA expression of Runx2, osteoprotegerin (OPG), and receptor activator of nuclear factor kappaBeta ligand (RANKL) by 60.49%, 72.74%, and 87.51%, respectively. Nevertheless, PRL did not change the RANKL/OPG ratio, since expression of OPG and RANKL were proportionally decreased. These concentrations of PRL had no effect on the mRNA expression of osteocalcin and osteopontin, nor on mineralization. High pathologic concentrations of PRL...Continue Reading

References

Nov 1, 1980·Molecular and Cellular Endocrinology·C WangG F Erickson
Dec 4, 1996·Biochemical and Biophysical Research Communications·N Bataille-SimoneauL Mercier
Apr 1, 1997·Endocrine Journal·N KrishnamraL Limlomwongse
Apr 16, 1998·The American Journal of Clinical Nutrition·L D RitchieJ C King
Nov 30, 2000·American Journal of Physiology. Endocrinology and Metabolism·D CossA M Walker
Jun 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N L FazzalariD M Findlay
Aug 3, 2001·American Journal of Obstetrics and Gynecology·S Honjo, H Mizunuma
May 6, 2003·Methods in Molecular Medicine·Astrid Bakker, Jenneke Klein-Nulend
Sep 25, 2003·Archives of Gynecology and Obstetrics·U UlrichM R Soules
Oct 31, 2003·Nature·Quyen Q HoangDaniel S C Yang
Nov 9, 2004·The American Journal of Clinical Nutrition·Flávia F BezerraCarmen M Donangelo
Dec 1, 2004·Calcified Tissue International·Basem M AbdallahBo Abrahamsen
Jan 29, 2005·Annals of the New York Academy of Sciences·Hong Liu-SeifertShaw Lamberson
Mar 8, 2005·Journal of Bone and Mineral Metabolism·Karine BertauxJean-Christophe Devedjian
Jul 26, 2005·Nature Medicine·Takako KogaHiroshi Takayanagi
Sep 29, 2005·Current Opinion in Pharmacology·Paul J Kostenuik
Jul 20, 2006·British Journal of Haematology·David N Haylock, Susan K Nilsson
Oct 5, 2006·The Journal of Clinical Psychiatry·Do-Un JungJoo-Cheol Shim
Jan 16, 2007·Canadian Journal of Physiology and Pharmacology·Supaporn PuntheeranurakNateetip Krishnamra
Mar 14, 2007·Experimental Cell Research·Elisabetta LambertiniRoberta Piva
Sep 8, 2007·Canadian Journal of Physiology and Pharmacology·Narattaphol Charoenphandhu, Nateetip Krishnamra

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Citations

Sep 18, 2009·The Journal of Physiological Sciences : JPS·Panan SuntornsaratoonNarattaphol Charoenphandhu
Mar 10, 2011·Expert Opinion on Drug Safety·Simon Matthew GrahamEvangelia Tsapakis
Jul 21, 2012·General and Comparative Endocrinology·Kannikar Wongdee, Narattaphol Charoenphandhu
Dec 29, 2009·Experimental and Molecular Pathology·Kannikar WongdeeNarattaphol Charoenphandhu
Oct 15, 2009·Cell Biochemistry and Function·Amporn NuntapornsakNarattaphol Charoenphandhu
Mar 31, 2016·Cellular and Molecular Life Sciences : CMLS·Carmen ClappGonzalo Martínez de la Escalera
Nov 17, 2016·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Muyesser Sayki ArslanErman Cakal
Oct 30, 2013·Therapeutic Advances in Psychopharmacology·Chadi A CalargeJanet A Schlechte
Jan 13, 2016·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Abbas Ali GaeiniSeyedeh Neda Mousavi
May 8, 2019·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Ming ChenShi-Ting Li
Jul 25, 2019·Animal : an International Journal of Animal Bioscience·L E Hernández-CastellanoR M Bruckmaier
Dec 1, 2010·Molecular and Cellular Biochemistry·Kannikar WongdeeNarattaphol Charoenphandhu
Oct 29, 2020·Seminars in Cell & Developmental Biology·Ödül Karayazi AtıcıCarrie S Shemanko

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