PMID: 7011046Mar 1, 1981Paper

Prolactin effects on ion transport across cultured mouse mammary epithelium

The American Journal of Physiology
C A Bisbee

Abstract

Prolactin is a known osmoregulatory hormone in lower vertebrates, and recent evidence indicates that this hormone modulates ionic concentrations in milk. In an ultrastructurally and biochemically differentiated primary cell culture system in which mouse mammary epithelium is maintained on floating collagen gels, prolactin causes an increase in short-circuit current (Isc) of monolayers of cells derived from midpregnant (24.6 to 48.0 microA . cm-2) and lactating (10.4 to 16.1 microA . cm-2) glands. Transepithelial potential differences (basal side ground) average about -12 mV and are similar to those seen in vivo. Prelactating mammary epithelial cell cultures have transepithelial resistances ranging from 374 omega . cm2 (prolactin present) to 507 omega . cm2 (prolactin absent), and lactating cell cultures have resistances averaging almost 1,000 omega . cm2. Prolactin effects require at most one day of culture maintenance in prolactin-containing medium, and the effects are not due to known contamination of prolactin preparations with arginine vasopressin or growth hormone. Medium concentrations of prolactin as low as 1 ng/ml can elicit these effects. In prelactating cell cultures not treated with prolactin, the Isc is equal to the...Continue Reading

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Citations

Jun 1, 1987·Pflügers Archiv : European journal of physiology·L BussieresC Sachs
Apr 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·P S Pedersen
Aug 19, 2007·American Journal of Physiology. Cell Physiology·Shivaram SelvamSamuel C Yiu
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Jan 1, 1983·Critical Reviews in Clinical Laboratory Sciences·A D Macknight, J P Leader

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