PMID: 7031659Nov 1, 1981Paper

Prolactin induces release of a factor from membranes capable of stimulating beta-casein gene transcription in isolated mammary cell nuclei

Proceedings of the National Academy of Sciences of the United States of America
B TeyssotJ Djiane

Abstract

Crude microsomes from lactating rabbit mammary gland were incubated with prolactin. The incubation mixture was centrifuged and the supernatant was incubated with isolated mammary cell nuclei from lactating rabbits treated for 4 days by bromocryptin to antagonize prolactin and to deinduce casein gene transcription. Nuclei were incubated with HgCTP, and the newly synthesized mercurated RNA was isolated on SH-Sepharose columns. The content of beta-casein mRNA sequences in the fraction eluted with 2-mercaptoethanol was estimated with a [(3)H]cDNA probe obtained from partially purified beta-casein mRNA. The supernatant markedly stimulated beta-casein gene transcription but not 28S rRNA transcription. The same effect was obtained with other lactogenic hormones such as human growth hormone and ovine placental lactogen but was not observed with bovine growth hormone, insulin, parathyroid hormone, luteotropic hormone, or epidermal growth factor. Prolactin and human growth hormone were totally inactive when added directly to nuclei. The factor stimulating beta-casein gene transcription was also generated by membranes containing prolactin receptors such as those from liver, ovary, adrenals, and brain but not by membranes from heart, lung,...Continue Reading

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P M TerryR M Lui
Apr 11, 1977·Biochemical and Biophysical Research Communications·M Zasloff, G Felsenfeld
Jan 1, 1979·Nucleic Acids Research·G F CrouseP Doty
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Sep 1, 1980·European Journal of Biochemistry·B Teyssot, L M Houdebine
Nov 28, 1980·Biochemical and Biophysical Research Communications·B Teyssot, L M Houdebine

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Citations

Jan 14, 1983·Biochemical and Biophysical Research Communications·G HuezB Lebleu
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J Silverthorne, E M Tobin
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L M HoudebineP A Kelly
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M Ben-DavidA V Schally
Oct 18, 1986·British Medical Journal·K Dickerson, P Hewitt
Feb 13, 1984·Life Sciences·R Re, M Parab
Nov 1, 1982·European Journal of Cancer & Clinical Oncology·J BonneterreP Cappelaere
Jan 1, 1986·Annals of the New York Academy of Sciences·J M RosenL Y Yu-Lee
Mar 1, 1983·Clinics in Endocrinology and Metabolism·M J Cronin, W S Evans
Feb 1, 1985·Journal of Dairy Science·B K VonderhaarT M Horn
Feb 1, 1985·Journal of Dairy Science·L M HoudebineJ L Servely
Jun 1, 1983·Journal of Dairy Science·P V Malven, T W Keenan
Mar 1, 1984·Journal of Cellular Physiology·M E Costlow, A Hample
Jul 1, 1983·Scottish Medical Journal·A P Forrest, R A Hawkins
Jan 1, 1985·The American Journal of Physiology·M DunandB C Rossier

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