Prolactin inhibits the apoptosis of chondrocytes induced by serum starvation

The Journal of Endocrinology
C ZermeñoCarmen Clapp

Abstract

The apoptosis of chondrocytes plays an important role in endochondral bone formation and in cartilage degradation during aging and disease. Prolactin (PRL) is produced in chondrocytes and is known to promote the survival of various cell types. Here we show that articular chondrocytes from rat postpubescent and adult cartilage express the long form of the PRL receptor as revealed by immunohistochemistry of cartilage sections and by RT-PCR and Western blot analyses of the isolated chondrocytes. Furthermore, we demonstrate that PRL inhibits the apoptosis of these same chondrocytes cultured in low-serum. Chondrocyte apoptosis was measured by hypodiploid DNA content determined by flow cytometry and by DNA fragmentation evaluated by the ELISA and the TUNEL methods. The anti-apoptotic effect of PRL was dose-dependent and was prevented by heat inactivation. These data demonstrate that PRL can act as a survival factor for chondrocytes and that it has potential preventive and therapeutic value in arthropathies characterized by cartilage degradation.

Citations

Aug 3, 2013·The Journal of Clinical Investigation·Norma AdánCarmen Clapp
Oct 5, 2010·Histochemistry and Cell Biology·Panan SuntornsaratoonNarattaphol Charoenphandhu
May 21, 2013·General and Comparative Endocrinology·Maricela LunaCarlos Arámburo
Aug 2, 2015·General and Comparative Endocrinology·José Luis Luna-AcostaCarlos Arámburo
Aug 29, 2009·Arthritis and Rheumatism·Koji TakayamaRyosuke Kuroda
Mar 13, 2012·Biochemical and Biophysical Research Communications·Dutmanee SeriwatanachaiNarattaphol Charoenphandhu
Mar 31, 2016·Cellular and Molecular Life Sciences : CMLS·Carmen ClappGonzalo Martínez de la Escalera
Feb 11, 2014·General and Comparative Endocrinology·Norma AdánCarmen Clapp
Feb 27, 2015·Journal of Inflammation·Joseph M McCoyKimberlee S Mix
Dec 3, 2014·Autoimmunity Reviews·Massimo CostanzaRosetta Pedotti
Apr 29, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Georgina OrtizCarmen Clapp

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis