Prolactin promotes the secretion of active cathepsin D at the basal side of rat mammary acini.

Endocrinology
Roberta CastinoM Ollivier-Bousquet

Abstract

Cathepsin D (CD), a lysosomal aspartic protease present in mammary tissue and milk in various molecular forms, is also found in the incubation medium of mammary acini in molecular forms that are proteolytically active on prolactin at a physiological pH. Because prolactin controls the vesicular traffic in mammary cells, we studied, in vivo and in vitro, its effects on the polarized transport and secretion of various forms of CD in the rat mammary gland. CD accumulated in vesicles not involved in endocytosis in the basal region of cells. Prolactin increased this accumulation and the release of endosomal active single-chain CD at the basal side of acini. The CD-mediated proteolysis of prolactin, leading to the antiangiogenic 16-kDa form, at a physiological pH, was observed only in conditioned medium but not milk. These data support the novel concept that an active molecular form of CD, secreted at the basal side of the mammary epithelium, participates in processing blood-borne prolactin outside the cell, this polarized secretion being controlled by prolactin itself.

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Citations

Nov 28, 2012·Cell and Tissue Research·Serge DelpalMichèle Ollivier-Bousquet
May 2, 2009·Journal of Mammary Gland Biology and Neoplasia·Jenifer Monks, Peter M Henson
May 14, 2009·Journal of Mammary Gland Biology and Neoplasia·Christine J Watson, Peter A Kreuzaler
Aug 14, 2010·Journal of Mammary Gland Biology and Neoplasia·Anne-Catherine Andres, Valentin Djonov
Dec 1, 2009·Animal : an International Journal of Animal Bioscience·S Truchet, M Ollivier-Bousquet
Dec 29, 2010·Médecine sciences : M/S·Mustapha LkhiderMichèle Ollivier-Bousquet
Mar 7, 2014·Journal of Molecular Endocrinology·Michiyo IshidaToshio Harigaya
Jul 29, 2008·Journal of Cellular and Molecular Medicine·Roberta CastinoCiro Isidoro
Jul 22, 2010·Cancer Biology & Therapy·Evette S Radisky
Nov 26, 2016·BMC Veterinary Research·Alessandra CrisàBianca Moioli

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