Prolactin-regulated tyrosine hydroxylase activity and messenger ribonucleic acid expression in mediobasal hypothalamic cultures: the differential role of specific protein kinases

Endocrinology
Frank Y MaStephen J Bunn

Abstract

Prolactin secretion from the anterior pituitary is tightly regulated by feedback onto the hypothalamic neuroendocrine dopaminergic (NEDA) neurons. Prolactin stimulates these neurons to synthesize and secrete dopamine, which acts via the pituitary portal vasculature to inhibit prolactin secretion from the pituitary lactotrophs. Despite the physiological importance of this feedback, relatively little is known about the signaling mechanisms responsible for prolactin activation of NEDA neurons. This issue has been examined here using a cell culture preparation of the fetal rat mediobasal hypothalamus. Prolactin stimulated a time- and concentration-dependent increase in catecholamine synthesis, which was maximal after 60-120 min (1 microg/ml prolactin) and inhibited by the prolactin antagonist Delta1-9-G129R-hPRL. This prolactin response was accompanied by a rise in the site-specific (ser-19, -31, and -40) phosphorylation of tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine synthesis. Consistent with this observation, the prolactin-induced increase in catecholamine synthesis was abolished by inhibitors of protein kinase A and protein kinase C (PKC). Prolactin incubation also resulted in a PKC-dependent activation ...Continue Reading

Citations

Feb 10, 2006·American Journal of Physiology. Endocrinology and Metabolism·Marcel EgliMarc E Freeman
Dec 7, 2007·Endocrine Reviews·Nira Ben-JonathanElizabeth W LaPensee
May 27, 2014·PloS One·Jimena FerrarisDaniel Pisera
Feb 25, 2014·Frontiers in Neuroendocrinology·David J Lyons, Christian Broberger
Jan 26, 2008·Journal of Mammary Gland Biology and Neoplasia·Estelle TalletVincent Goffin
Feb 16, 2012·Gender Medicine·Evdoxia TsigkaropoulouLefteris Lykouras
Aug 9, 2011·Brain, Behavior, and Immunity·C M Larsen, D R Grattan
Feb 13, 2008·Journal of Neuroendocrinology·David R GrattanStephen J Bunn
Jul 8, 2008·Journal of Neuroendocrinology·D R Grattan, I C Kokay
Aug 20, 2011·Journal of Neuroendocrinology·A Sirzen-ZelenskayaM Egli
Jun 24, 2015·The Journal of Endocrinology·David R Grattan
Apr 25, 2014·Channels·Mayur J PatilArmen N Akopian
Aug 20, 2010·Biology of Reproduction·David J HodsonDomingo J Tortonese
Oct 7, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Vincent GoffinPaul A Kelly
Jan 18, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·I C KokayD R Grattan
Sep 6, 2007·The Journal of Biological Chemistry·Jean-Baptiste JomainVincent Goffin
Aug 10, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·María Abellán-ÁlvaroEnrique Lanuza

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