Prolactin-releasing factor: cellular origin in the intermediate lobe of the pituitary

Endocrinology
M LaudonN Ben-Jonathan

Abstract

Our laboratory has provided substantial evidence for the presence of PRL-releasing factor (PRF) in the posterior pituitary. The objectives of this study were 1) to determine the distribution of PRF activity between the neural and intermediate lobes, and 2) to assess the PRF activity of cultured posterior pituitary cells. Posterior pituitaries from adult male rats were dispersed with trypsin and cultured for 1-7 days. Cultured cells or intact posterior pituitaries were extracted with acid and lyophilized. PRF activity was determined by the ability of reconstituted extracts to increase PRL release from cultured anterior pituitary cells. Upon dissection of the posterior pituitary, PRF activity was primarily present in the intermediate lobe. There was minimal contamination between the two lobes, as indicated by the localization of 90% of the total oxytocin in the neural lobe and 95% of alpha MSH in the intermediate lobe. Extracts from intact posterior pituitaries and posterior pituitary cells cultured for 4 days stimulated PRL secretion in a similar dose-dependent manner. Cultured liver and cerebral cortex cells had very low PRF activity. Both oxytocin and dopamine, two neuronal markers, were reduced to less than 5% of their origin...Continue Reading

Citations

Mar 1, 1992·Molecular and Cellular Endocrinology·T E PorterL S Frawley
Jan 1, 1997·Brain Research Bulletin·M Juszczak, B Stempniak
Apr 1, 1994·Trends in Endocrinology and Metabolism : TEM·L Stephen Frawley
May 13, 2006·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·John J Orrego, John Bair
Jul 9, 2005·Molecular Endocrinology·Chia Lin ChangSheau Yu Teddy Hsu
Oct 4, 2000·Physiological Reviews·M E FreemanG Nagy

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