Proliferating bloodstream-form Trypanosoma brucei use a negligible part of consumed glucose for anabolic processes

International Journal for Parasitology
Jurgen R HaanstraBarbara M Bakker

Abstract

Our quantitative knowledge of carbon fluxes in the long slender bloodstream form (BSF) Trypanosoma brucei is mainly based on non-proliferating parasites, isolated from laboratory animals and kept in buffers. In this paper we present a carbon balance for exponentially growing bloodstream form trypanosomes. The cells grew with a doubling time of 5.3h, contained 46 μ mol of carbon (10(8) cells)(-1) and had a glucose consumption flux of 160 nmol min(-1) (10(8) cells)(-1). The molar ratio of pyruvate excreted versus glucose consumed was 2.1. Furthermore, analysis of the (13)C label distribution in pyruvate in (13)C-glucose incubations of exponentially growing trypanosomes showed that glucose was the sole substrate for pyruvate production. We conclude that the glucose metabolised in glycolysis was hardly, if at all, used for biosynthetic processes. Carbon flux through glycolysis in exponentially growing trypanosomes was 10 times higher than the incorporation of carbon into biomass. This biosynthetic carbon is derived from other precursors present in the nutrient rich growth medium. Furthermore, we found that the glycolytic flux was unaltered when the culture went into stationary phase, suggesting that most of the ATP produced in glyc...Continue Reading

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Citations

Apr 11, 2013·Antimicrobial Agents and Chemotherapy·Darren J CreekMichael P Barrett
Dec 18, 2013·PLoS Computational Biology·Eduard J KerkhovenMichael P Barrett
Oct 23, 2015·Metabolomics : Official Journal of the Metabolomic Society·Dong-Hyun KimMichael P Barrett
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Mar 8, 2014·Molecular and Biochemical Parasitology·Amaranta Gómez-ArreazaLuisana Avilán
Dec 6, 2014·Molecular and Biochemical Parasitology·Jurgen R HaanstraPaul A M Michels
Jan 14, 2017·Scientific Reports·Jurgen R HaanstraBarbara M Bakker
Jun 11, 2015·Memórias do Instituto Oswaldo Cruz·S Andrea Moreno, Mayerly Nava
Jun 25, 2017·Molecular and Biochemical Parasitology·Rocío Rondón-MercadoJuan Luis Concepción
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Apr 14, 2016·PLoS Neglected Tropical Diseases·Diego BenítezMarcelo A Comini
Jul 2, 2014·Biochemical and Biophysical Research Communications·Elizabeth A BlackburnMalcolm D Walkinshaw

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