Proliferating cell nuclear antigen (pol30) mutations suppress cdc44 mutations and identify potential regions of interaction between the two encoded proteins.

Molecular and Cellular Biology
M A McAlearC Holm

Abstract

In addition to its role as a processivity factor in DNA replication, proliferating cell nuclear antigen (PCNA) may function in the regulation of cell cycle progression. We present genetic evidence that PCNA interacts with the gene product of CDC44, an essential nucleotide-binding protein that encodes the large subunit of yeast replication factor C (K. Fien and B. Stillman, personal communication). Mutations in POL30 (PCNA) suppress cold-sensitive alleles of cdc44 that contain mutations in or near nucleotide-binding consensus domains, but they do not suppress a null allele. Thus, it appears that PCNA interacts with Cdc44p but cannot substitute for its function. pol30 mutations suppress additional phenotypes of cdc44 mutations, including the cold sensitivity that they were selected to suppress. This observation suggests an intimate association between PCNA and Cdc44p. Each of five independent pol30 mutants contains a unique single mutation that maps to a localized region on one face of the predicted three-dimensional structure of PCNA. This face identifies a region likely to be important for functional interaction between the CDC44 and POL30 gene products.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1979·Journal of General Microbiology·K J Hutter, H E Eipel
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J Jarvik, D Botstein
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M ChenJ Hurwitz
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M ChenJ Hurwitz
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·A G So, K M Downey
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S H Lee, J Hurwitz
Sep 21, 1990·Cell·A MorrisonA Sugino
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M S Wold, T Kelly
Apr 2, 1987·Nature·R BravoH Macdonald-Bravo
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S H LeeJ Hurwitz
Sep 1, 1985·Microbiological Reviews·R K Mortimer, D Schild
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·U B Gyllensten, H A Erlich
Nov 1, 1985·Cell·L M JohnsonJ L Campbell
Mar 1, 1980·The Journal of Cell Biology·R Bravo, J E Celis
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Li, P M Burgers
Jan 11, 1993·Nucleic Acids Research·M O'DonnellJ Hurwitz

❮ Previous
Next ❯

Citations

Aug 31, 2000·Annual Review of Biochemistry·T A Kunkel, K Bebenek
Oct 6, 1998·Annual Review of Biochemistry·S Waga, B Stillman
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·D J MozzherinP A Fisher
May 15, 2001·Journal of Molecular Biology·A Schepers, J F Diffley
Sep 1, 2019·The Biochemical Journal·Juan Alejandro PerdomoElizabete Carmo-Silva
Jan 17, 1997·The Journal of Biological Chemistry·R MossiU Hübscher
Apr 5, 2001·Molecular and Cellular Biology·J S HannaF A Spencer
May 4, 2005·The Journal of Biological Chemistry·Cishan LiArchie R Portis
Aug 24, 1999·Methods : a Companion to Methods in Enzymology·M P Arroyo, T S Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.