Proliferation and apoptosis of follicular lymphocytes: relationship to follicular dendritic cell-associated clusters

Immunology
H OruiY Imai

Abstract

Experiments were designed to determine the in vivo cell-cycle phase of lymphocytes in secondary lymphoid follicles, and whether B-cell proliferation and apoptosis occur within follicular dendritic cell (FDC)-associated clusters. Using frozen serial sections of human tonsils, lymphoid follicles were stained to reveal histone H3 mRNA, as an S-phase marker, using in situ hybridization, and stained immunohistochemically with antibodies against cyclin E as a late G1 phase marker, cyclin B1 and p34cdc2 as S-G2-M phase markers, and Ki-67 as a marker of cycling cells. Each LF was divided into five zones: mantle zone, outer zone, apical light zone, basal light zone and dark zone, with the help of haematoxylin and eosin staining, and a CD23 immunostain. The rate of occurrence of positively labelled cells was calculated by dividing the number of positive cells by the number of all cells in each zone. The cells that were positive for cyclin E, histone H3 mRNA, cyclin B1, p34cdc2, and Ki-67 were found most frequently in the dark zone (54.5 +/- 6.6%, 22.0 +/- 5.7%, 36.7 +/- 14.5%, 40.0 +/- 10.2%, and 59.0 +/- 13.4%, respectively), followed by the outer zone (52.7 +/- 7.8%, 14.9 +/- 4.1%, 22.9 +/- 9.7%, 24.9 +/- 7.9%, and 44.6 +/- 12.3%, resp...Continue Reading

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Citations

Nov 5, 1999·The Journal of Pathology·C S VerbekeH Zentgraf
Mar 7, 2006·Clinical Rheumatology·Chikako Takahashi TohyamaHajime Ishikawa
Dec 4, 2003·International Journal of Pediatric Otorhinolaryngology·M MusiatowiczM Baltaziak
Feb 24, 2012·PLoS Pathogens·Rocio Castro-SeoanePeter-Christian Klöhn
May 29, 2009·Journal of Clinical and Experimental Hematopathology : JCEH·Hirofumi MisakiMitsunori Yamakawa
Feb 24, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W S HlavacekA S Perelson

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