Proliferation and apoptosis of HeLa cells induced by in vitro stimulation with digitalis

European Journal of Pharmacology
Margarita Ramirez-OrtegaGuillermo Ceballos-Reyes

Abstract

In the HeLa tumor cell line, we studied the characteristics of the dual effect of digitalis compounds on cell growth (proliferation and death). In addition, we explored whether both effects occur by means of the same mechanism. HeLa cell cultures were exposed to increasing concentrations (0.01 nM-10 microM) of ouabain, strophantidin, digoxin, and digoxigenin at 24-96 h intervals. Cell growth in treated cultures was compared with cell growth under nontreated conditions. Additionally, we studied changes in nuclear morphology, as well as in genomic DNA degradation, cytochrome c release, and caspase-9 and -3 presence and processing induced by toxic concentrations of digitalis. Digitalis compounds increased HeLa cell number when exposed to concentrations <10 nM during a 48 h period. Ethacrynic acid (a nonsteroid inhibitor for Na+/K+-ATPase) did not induce cell growth at these concentrations. Digitalis concentrations >10 nM induced cell death in a concentration- and exposure period-dependent fashion. Changes in nuclear morphology, DNA fragmentation, mitochondrial cytochrome c release, and proteolytic processing of caspases-9 and -3, suggest apoptotic cell death. The IC50 for the inducing effect of apoptosis by ouabain at 96 h was 18 ...Continue Reading

References

Oct 1, 1989·Analytical Biochemistry·W KuengU Eppenberger
Dec 11, 1994·Nucleic Acids Research·M HerrmannJ R Kalden
Jul 8, 1998·Bioscience Reports·B OlejV M Rumjanek
Jun 8, 2001·Anti-cancer Drugs·S JohanssonP Claeson
Oct 2, 2001·The Journal of Biological Chemistry·A Aydemir-KoksoyJ C Allen
Dec 1, 2001·Nature Medicine·G Scheiner-Bobis, W Schoner
May 25, 2002·European Journal of Biochemistry·Zijian Xie, Amir Askari
May 9, 2003·The Journal of Biological Chemistry·Renata I Dmitrieva, Peter A Doris
May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·R Takasawa, S Tanuma

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Citations

Feb 20, 2008·Pflügers Archiv : European journal of physiology·Zhichuan Li, Zijian Xie
Sep 1, 2009·World Journal of Pediatrics : WJP·Jia-Wei XuYan Bai
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Isabel LarreMarcelino Cereijido
Feb 3, 2007·Biological & Pharmaceutical Bulletin·Jie QiuMei Cheng
Feb 3, 2009·Cancer Chemotherapy and Pharmacology·Elena BloiseMaria Antonietta Belisario
May 15, 2009·The American Journal of the Medical Sciences·M Iltaf KhanDonald M Miller
Jun 28, 2012·Mitochondrion·Claudia CerellaMarc Diederich
Feb 1, 2011·Pharmacology & Therapeutics·Christos VaklavasApostolia Maria Tsimberidou
Oct 25, 2008·Toxicon : Official Journal of the International Society on Toxinology·Damien C T HallidayAlex Hyatt
May 1, 2007·European Journal of Pharmacology·Margarita Ramirez-OrtegaGustavo Pastelin
Mar 15, 2016·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Emanuela PesceLuis J V Galietta
Dec 11, 2013·IUBMB Life·Jeffrey X XieZijian Xie
Nov 17, 2012·Journal of Experimental & Clinical Cancer Research : CR·Francesca CuozzoLivia Di Renzo
May 26, 2007·Biochimica Et Biophysica Acta·Andrey KulikovGeorgios Scheiner-Bobis
Aug 23, 2016·Journal of Ethnopharmacology·L A Rascón-ValenzuelaR E Robles-Zepeda
Oct 7, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yucheng ShenYe Tian
Nov 9, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Naira Fernanda Zanchett SchneiderMarc Diederich
Jun 15, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoyu Cui, Zijian Xie
Jul 30, 2008·British Journal of Clinical Pharmacology·Edward J Calabrese
Oct 20, 2006·American Journal of Physiology. Cell Physiology·Elena ArystarkhovaKathleen J Sweadner
May 15, 2007·American Journal of Physiology. Cell Physiology·Wilhelm Schoner, Georgios Scheiner-Bobis
Jul 17, 2019·Journal of Cellular Biochemistry·Bruno de Souza GonçalvesHérica de Lima Santos
Mar 23, 2017·Cytotechnology·Júlia Teixeira de OliveiraFabio Vieira Dos Santos
Mar 25, 2020·Dose-response : a Publication of International Hormesis Society·Ainy ZehraZaigham Abbas
Mar 20, 2021·Integrative Cancer Therapies·Jou-Chun ChouPaulus S Wang

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