Proliferation and committed differentiation into dopamine neurons of neural stem cells induced by the active ingredients of radix astragali

Neuroreport
Han GaoWentao Li

Abstract

Neural stem cells (NSCs) are important cellular sources of transplantation therapies for Parkinson's disease. This study aimed to determine the effects of extracts of radix astragali on the proliferation and differentiation into dopamine (DA) neurons in NSCs. NSCs were dealt with astragaloside IV (ASI), astragalus polysaccharide (APS), and astraisoflavan (ASF), the main active ingredients of radix astragali. First, the results from cell-count kit-8 (CCK-8) assay showed that ASI, ASF, and APS had positive effects on the proliferation of NSCs. Next, we also confirmed the effects of ASI, APS, and ASF on BrdU and nestin by immunocytochemistry. Moreover, results from quantitative RT-PCR showed ASI, APS, and ASF could promote the expressions of tyrosine hydroxylase and dopamine transporter mRNA, which are specifically expressed in DA neurons. Simultaneously, sonic hedgehog (Shh), orphan nuclear hormone 1 (Nurr1), and pituitary homeobox 3 (Ptx3) are considered to motivate the formation of DA neurons. Our result showed ASI, APS, and ASF can also promote the expressions of Shh, Nurr1, and Ptx3 mRNAs. In conclusion, our study verifies that the active ingredients of radix astragali can promote the proliferation of NSCs and induce NSC diff...Continue Reading

References

Nov 19, 2003·European Journal of Pharmacology·Marten P SmidtJ Peter H Burbach
Oct 6, 2006·Nucleic Acids Research·Paola SacchettiPhilippe Lefebvre
Dec 23, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Kirsten MessmerPaul S Fishman
Feb 26, 2008·Nature Neuroscience·Young-Goo HanArturo Alvarez-Buylla
Sep 11, 2014·Neural Regeneration Research·Jonghyuck ParkRiyi Shi
Sep 17, 2014·Trends in Neurosciences·Daniel A Lim, Arturo Alvarez-Buylla

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Citations

Jul 30, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Chen WangJin-Wen Xu
Dec 15, 2020·The American Journal of Chinese Medicine·Yanleng HuangLan Shen
May 14, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Sicong LiXin Liu
Jun 28, 2021·Journal of Ethnopharmacology·Junying DingQingquan Liu

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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS
GraphPad Prism
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GraphPad
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