Proliferation and differentiation of neural stem cells on lysine-alanine sequential polymer substrates

Biomaterials
Jyh-Horng WangTai-Horng Young

Abstract

The purpose of this study was to explore the phenotypic potential of embryonic rat cerebral cortical stem cells by inducing differentiation on lysine-alanine sequential (LAS) polymer substrates at neurosphere level. LAS polymer is a heterologous polymer of lysine and alanine and has been demonstrated to enhance axon growth of neurons in a serum-free medium in vitro. It was found that very few cells migrated outside of the neurospheres but extremely long processes extended from differentiated cells could form a network between remote neurospheres when cells were cultured on LAS substrates at a low density of 120 neurospheres/cm(2) in the serum-free medium. On the contrary, when the neurosphere density was increased to 360 neurospheres/cm(2), many neurosphere-forming cells migrated out from their original aggregate and exhibited short processes morphology. Furthermore, when serum was added to the culture system, the neurosphere-forming cells could be induced into an extensive cellular substratum of protoplasmic cells upon which process-bearing cells spread. Clearly, neurospheres could exhibit different behaviors on LAS substrates according to the complex environmental conditions. Here, we proposed that neurospheres would change t...Continue Reading

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Citations

Jul 3, 2010·Science China. Life Sciences·ZhaoYang YangXiaoGuang Li
Jan 19, 2012·Biomacromolecules·Lei CaiShanfeng Wang
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Feb 16, 2010·Biomaterials·Xian-Yi XuGuo-Qiang Chen
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Dec 17, 2014·Journal of Biomaterials Applications·I-Chi LeeWen-Ting Yang
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