PMID: 6983069Oct 1, 1982Paper

Proliferation and differentiation of single hapten-specific B lymphocytes is promoted by T-cell factor(s) distinct from T-cell growth factor

Proceedings of the National Academy of Sciences of the United States of America
B L PikeG J Nossal

Abstract

Hapten-specific B lymphocytes reactive to fluorescein were prepared from mouse spleen, placed singly in 10-microliters culture wells, and stimulated with fluorescein-polymerized flagellin in the presence of conditioned media (CM) from various concanavalin A-stimulated cloned T-cell tumors or hybridomas. Antigen plus appropriate CM triggered 5-9% of the B cells into both clonal proliferation and differentiation into antibody-forming cells. Antigen alone stimulated 0.5-0.8% of B cells and CM alone stimulated less than 0.1%. This bioactivity was termed B-cell growth and differentiation factor(s) (BGDF). Four CM rich in T-cell growth factor (TCGF)--namely, CM from spleen and the lines EL4, T6, and 123--contained BGDF. The lines T19.1 and WEHI-3 lacked BGDF and TCGF. Four lines of evidence suggested that BGDF and TCGF were distinct molecules. First, the BGDF/TCGF ratios in the various CM varied. Second, on gel filtration, TCGF eluted as a sharp peak corresponding to a Mr of about 35,000, whereas BGDF eluted over a range corresponding to a Mr of 25,000-60,000. Third, the activity of TCGF in EL4-CM was markedly reduced by treatment with guanidine HCl while BGDF activity was not. Fourth, BGDF showed more heterogeneity than TCGF on hydr...Continue Reading

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Citations

Jul 1, 1987·Journal of Clinical Immunology·T MiyawakiM D Cooper
Jan 1, 1985·Folia Microbiologica·G J Nossal
Apr 21, 1989·Journal of Immunological Methods·M G McHeyzer-Williams, G J Nossal
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M L JelachichS K Pierce
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B L PikeG J Nossal
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G S HebbardG J Nossal
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B L Pike, G J Nossal
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T HiranoT Kishimoto
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B L Pike, G J Nossal
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J J MondR J Robb
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N HaradaT Honjo
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M S LoughnanG J Nossal
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M R AldersonG J Nossal
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M S LoughnanG J Nossal
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G J Nossal, M Karvelas
Oct 1, 1984·The Journal of Experimental Medicine·R H ZublerH R MacDonald
Nov 1, 1984·The Journal of Experimental Medicine·T A WaldmannW C Greene
Sep 1, 1984·Annales d'immunologie·G J Nossal
Jan 1, 1990·Leukemia Research·C G BegleyT A Waldmann
Jan 1, 1986·Annals of the New York Academy of Sciences·Y J RosenbergW Ohriner
Aug 22, 1986·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·G J Nossal
Apr 11, 1991·Nature·M G McHeyzer-WilliamsP A Lalor
Apr 1, 1984·Immunological Reviews·G J Prud'hommeA N Theofilopoulos
Apr 1, 1984·Immunological Reviews·M HowardW E Paul
Jun 1, 1985·Scandinavian Journal of Immunology·E RuuthE Lundgren
Nov 1, 1989·European Journal of Immunology·M G McHeyzer-Williams
Nov 1, 1986·Immunological Investigations·E L MorganW O Weigle
Jul 30, 2021·Frontiers in Immunology·Daniel BodeDavid G Kent

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