PMID: 9423716Jan 10, 1998Paper

Proliferation and extracellular matrix production by human infragenicular smooth muscle cells in response to interleukin-1 beta

Journal of Vascular Surgery
E A ForsythAnton N Sidawy

Abstract

Atherosclerotic peripheral vascular disease commonly involves the infragenicular arterial tree. Our study evaluated the effect of interleukin (IL)-1 beta on the proliferation of vascular smooth muscle cells (VSMCs) derived from atherosclerotic infragenicular arteries of human subjects who underwent below-knee amputation, as well as the role of IL-1 beta in VSMCs' production of extracellular matrix components, substances that are important in the transformation of VSMCs from the contractile to the synthetic phenotype. This transformation to the synthetic phenotype is an important step in the formation of the atherosclerotic lesion. Cultures were identified as being of smooth muscle origin through staining with the cytoskeletal marker, alpha-smooth muscle actin. Proliferation assays were performed by seeding confluent cultures of passages 4 to 7 into six-well plates at 10,000 cells per well. After serum starvation, samples were incubated with IL-1 beta (1 ng/ml). Cell number was determined on a daily basis. To study extracellular matrix production, cells were propagated in tissue culture chamber slides in the absence or presence of growth media containing IL-1 beta. After fixation with 100% methanol, each sample was stained with ...Continue Reading

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Citations

Dec 11, 2013·Translational Stroke Research·Norihito Shimamura, Hiroki Ohkuma
Aug 10, 2004·Surgery·Zhihua JiangC Keith Ozaki
Aug 11, 2007·Biomaterials·Peter ZillaPaul Human
Apr 6, 2002·International Immunology·Thomas LuftEugene Maraskovsky
Dec 17, 2020·Frontiers in Immunology·Vitaly SorokinCatherine M Shanahan

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