Abstract
Recent research has shown that TOP2A plays an important role in the tumorigenesis of many malignancies, such as breast cancer, ovarian cancer, and prostate cancer. However, few studies have been conducted on TOP2A expression and functions in colon cancer. In the present study, we found that TOP2A expression was obviously elevated in colon cancer tissues compared to adjacent non-cancerous tissues. Depletion of TOP2A in HCT116 and SW480 colon cancer cells by transfection of specific small interfering RNA significantly suppressed proliferation and inhibited invasion of cells, even induced apoptosis as indicated by both MTT assay, Annexin V/propidium iodide staining, and Transwell assay. Furthermore, we explored the underlying mechanisms. Knockdown of TOP2A not only affects the expression of cell apoptosis-related (Bcl-2 and Bax) and invasion-related proteins (MMP-2 and MMP-9), but also reduced the phosphorylation levels of ERK and AKT. In conclusion, we showed that TOP2A was upregulated in colon cancer tissue samples and that TOP2A may serve as an oncogene in colon cancer.
References
Nov 1, 1995·Current Opinion in Oncology·J C Reed
Jun 24, 2004·Seminars in Cell & Developmental Biology·Julian Downward
Feb 4, 2005·The New England Journal of Medicine·Jeffrey A Meyerhardt, Robert J Mayer
Jan 24, 2007·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Dec 21, 2007·Cardiovascular Research·Raghu AdyaHarpal S Randeva
Dec 17, 2008·Cancer Chemotherapy and Pharmacology·Nicolas TsavarisEfstratios Patsouris
Mar 17, 2009·International Journal of Oncology·Mathias Felix Leber, Thomas Efferth
May 29, 2009·Annals of Surgery·Po-Li WeiShyr-Yi Lin
Jan 25, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wen WenHongyang Wang
Mar 31, 2012·Cancer Research·Wen-Bin TsaiMacus Tien Kuo
Feb 13, 2013·Journal of Translational Medicine·Marina França de ResendeRafael Malagoli Rocha
Apr 9, 2013·Oncology Reports·Chan-Hun JungHong-Duck Um
Jul 23, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Gowrikumar SaiprasadGanapasam Sudhandiran
Dec 3, 2014·Cancer Letters·Shao-Lai ZhouJian Zhou
Jan 22, 2015·Oncotarget·Jason S KirkLeigh Ellis
Mar 8, 2016·Oncogene·Q ZhouD V LaBarbera
May 15, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marie KlintmanMitch Dowsett
Jul 14, 2016·Nucleic Acids Research·Kevin Z L WuCorrado Santocanale
Sep 23, 2016·European Journal of Medicinal Chemistry·Mohammad Shahidul IslamA F M Motiur Rahman
Nov 2, 2016·International Journal of Nanomedicine·Hongmei ZhengXinhong Wu
Jan 7, 2017·BMC Gastroenterology·Yongkuan CaoLin Zhang
Mar 31, 2017·Molecular Cancer·Jingkun ZhaoAiguo Lu
Aug 17, 2017·PloS One·Azadeh AmirnasrErik A C Wiemer
Aug 18, 2017·Medicine·Lu-Bing LiuFu-Ze Xin
Sep 14, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David P LabbéLeigh Ellis
Sep 15, 2017·The Journal of Pharmacology and Experimental Therapeutics·Steven R HallKerry B Goralski
Sep 17, 2017·Oncotarget·Masanori TerashimaUNKNOWN ACTS-GC Group
Jan 14, 2018·Breast Cancer Research and Treatment·Miriam Nuncia-CantareroEva Maria Galan-Moya
Citations
Mar 18, 2020·Cancers·Larissa Kotelevets, Eric Chastre
Jun 11, 2019·Molecular Medicine Reports·Xianchun DuanHuasheng Peng
Jul 30, 2020·The Journal of International Medical Research·Ziqi MengJingyuan Zhang
May 21, 2019·Frontiers in Genetics·Li LiuHongying He
Mar 24, 2020·The International Journal of Neuroscience·Gaowei LiLiangxue Zhou
Aug 28, 2020·Frontiers in Oncology·Zhihua XuYe Xu
Dec 26, 2018·Journal of Cellular Biochemistry·Peng-Fei ChenBin Fan
Jan 24, 2019·Frontiers in Oncology·Xiao-Ping LiuLi He
Sep 15, 2020·Chinese Medical Journal·Wen-Jie LyuPing Dong
Jun 26, 2020·Frontiers in Genetics·Weigang ChenZunnan Huang
Jan 1, 2020·Cancers·Lin PangXia Li
Dec 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingyi SongNingning He
Jun 18, 2020·Cancer Management and Research·Bi WangZhi Huang
Oct 18, 2020·International Journal of Molecular Sciences·Rachel A KeulsRonald J Parchem
Feb 3, 2021·IET Systems Biology·Zhi-Guang HuangSheng-Hua Li
Feb 7, 2021·Acta Pharmacologica Sinica·Peng ZengRu Zhang
Feb 6, 2021·Cancer Management and Research·Yuzi ZhaoLi Hong
Nov 1, 2020·Medicine·Jing-Jing DaiBing Wang
Dec 31, 2020·BioMed Research International·Yuqin TangXun Hu
Feb 16, 2021·Journal of Immunological Methods·Yulin WangLiping Dai
Mar 14, 2021·World Journal of Surgical Oncology·Renjie LiuDousheng Bai
Apr 4, 2021·International Journal of Molecular Sciences·Áron Bartha, Balázs Győrffy
Apr 3, 2021·Gene·Zhenxian JiaXuemei Zhang
Nov 7, 2020·Aging·Yutao WangJianbin Bi
May 23, 2021·Cell Death & Disease·Congcong ZhuYe Xu
Jul 23, 2021·International Journal of General Medicine·Mou-Chun GongFei-Hua Chen
Jul 21, 2021·Biochemical and Biophysical Research Communications·Fan Zhang, Hong Wu
Jul 25, 2021·Cancers·Haruka OmoriHitoshi Nagatsuka
Jul 29, 2021·Science Progress·Jihan WangWenjie Gao
Aug 13, 2021·Bioengineered·Gang ChenGuangdong Qiao
Nov 18, 2021·Bioengineered·Yi ZhouHaigang Li
Dec 24, 2021·Bioengineered·Yinying DongRuimei Ren