Proliferation and invasion of colon cancer cells are suppressed by knockdown of TOP2A

Journal of Cellular Biochemistry
Rui ZhangJing H Bai

Abstract

Recent research has shown that TOP2A plays an important role in the tumorigenesis of many malignancies, such as breast cancer, ovarian cancer, and prostate cancer. However, few studies have been conducted on TOP2A expression and functions in colon cancer. In the present study, we found that TOP2A expression was obviously elevated in colon cancer tissues compared to adjacent non-cancerous tissues. Depletion of TOP2A in HCT116 and SW480 colon cancer cells by transfection of specific small interfering RNA significantly suppressed proliferation and inhibited invasion of cells, even induced apoptosis as indicated by both MTT assay, Annexin V/propidium iodide staining, and Transwell assay. Furthermore, we explored the underlying mechanisms. Knockdown of TOP2A not only affects the expression of cell apoptosis-related (Bcl-2 and Bax) and invasion-related proteins (MMP-2 and MMP-9), but also reduced the phosphorylation levels of ERK and AKT. In conclusion, we showed that TOP2A was upregulated in colon cancer tissue samples and that TOP2A may serve as an oncogene in colon cancer.

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Citations

Mar 18, 2020·Cancers·Larissa Kotelevets, Eric Chastre
Mar 24, 2020·The International Journal of Neuroscience·Gaowei LiLiangxue Zhou
Dec 26, 2018·Journal of Cellular Biochemistry·Peng-Fei ChenBin Fan
Dec 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingyi SongNingning He
Oct 18, 2020·International Journal of Molecular Sciences·Rachel A KeulsRonald J Parchem
Mar 14, 2021·World Journal of Surgical Oncology·Renjie LiuDousheng Bai
Apr 4, 2021·International Journal of Molecular Sciences·Áron Bartha, Balázs Győrffy
Jul 23, 2021·International Journal of General Medicine·Mou-Chun GongFei-Hua Chen
Jul 21, 2021·Biochemical and Biophysical Research Communications·Fan Zhang, Hong Wu

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