Proliferation and Osteogenic Differentiation of hMSCs on Biomineralized Collagen

Frontiers in Bioengineering and Biotechnology
Daniel de Melo PereiraPamela Habibovic

Abstract

Biomineralized collagen with intrafibrillar calcium phosphate mineral provides an excellent mimic of the composition and structure of the extracellular matrix of bone, from nano- to micro-scale. Scaffolds prepared from this material have the potential to become the next-generation of synthetic bone graft substitutes, as their unique properties make them closer to the native tissue than synthetic alternatives currently available to clinicians. To understand the interaction between biomineralized collagen and cells that are relevant in the context of bone regeneration, we studied the growth and osteogenic differentiation of bone marrow derived human mesenchymal stromal cells (hMSCs) cultured on biomineralized collagen membranes, and compared it to the cell behavior on collagen membranes without mineral. Cells proliferated normally on both biomimetic membranes, and were more triggered to differentiate toward the osteogenic lineage by the biomineralized collagen. This was shown by the elevated mRNA levels of RUNX2, SPP1, ENPP1, and OCN after 3 days of culture, and COL1A1 after 14 days of culture on mineralized collagen. The mRNA levels of the tested markers of osteogenesis were lower on collagen membranes without mineral, with the ...Continue Reading

References

Jun 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L D QuarlesR J Wenstrup
Jun 1, 1989·The Anatomical Record·P V Hauschka, F H Wians
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R L Lees, J N Heersche
Dec 5, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·C M Giachelli, S Steitz
Jun 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lovisa HessleJosé Luis Millan
Jun 24, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kristen JohnsonRobert Terkeltaub
Jun 24, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Gerald J AtkinsAndrew C W Zannettino
May 1, 1951·The Journal of Experimental Medicine·R S SIFFERT
Apr 30, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·S S LiaoQ L Feng
Jun 24, 2006·Journal of Cellular Biochemistry·Toshihisa Komori
May 24, 2007·Tissue Engineering·Sanne K BothJoost D de Bruijn
Oct 28, 2009·Tissue Engineering. Part B, Reviews·Steven M van GaalenWouter J A Dhert
Dec 30, 2009·Tissue Engineering. Part a·Hugo FernandesJan de Boer
Jan 17, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Daniel O CostaAmin S Rizkalla
Jan 1, 2012·Faraday Discussions·Fabio NudelmanNico A J M Sommerdijk
Jun 10, 2016·ACS Applied Materials & Interfaces·Yu FuYan-Heng Zhou
Oct 26, 2016·Nature Materials·Ottman A Tertuliano, Julia R Greer
Aug 16, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Bihua YeChangren Zhou
Sep 27, 2016·Medical Science Monitor Basic Research·Arkady RutkovskiyIngvar Jarle Vaage
Jan 21, 2017·International Journal of Molecular Sciences·Yu-Tzu TsaoOscar K Lee
Sep 25, 2017·Journal of Functional Biomaterials·Elizabeth R KapasaXuebin B Yang
Apr 25, 2018·Journal of Tissue Engineering and Regenerative Medicine·Yao WangChangyi Li
May 5, 2018·Science·Natalie ReznikovRoland Kröger
Sep 22, 2018·Advanced Healthcare Materials·Daniel de Melo Pereira, Pamela Habibovic
May 3, 2019·Biomaterials·Ziryan OthmanPamela Habibovic

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Datasets Mentioned

BETA
GM

Methods Mentioned

BETA
Polymerase Chain Reaction
X-Ray
fluorescence microscopy

Software Mentioned

GraphPad Prism

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