Proliferation and progesterone production of ovine luteal cells from several stages of the estrous cycle: effects of fibroblast growth factors and luteinizing hormone

Canadian Journal of Physiology and Pharmacology
A T Grazul-BilskaL P Reynolds

Abstract

This study was conducted to evaluate the effects of fibroblast growth factor 1 (FGF-1), fibroblast growth factor 2 (FGF-2), or luteinizing hormone (LH) on proliferation and progesterone secretion of ovine luteal cells from days 5, 10, or 15 after estrus (estrus = day 0; n = 4 or 5 ewes/day). After enzymatic dispersion, luteal cells were incubated in the presence or absence of various doses of FGF-1, FGF-2, LH, or fetal bovine serum (FBS) (positive control) in serum-free media for 7 days in 24-well plates. Cells were counted on day 7 of culture and media analyzed for progesterone concentration. For all treatments, maximal effects (Emax) and dissociation constants (KD) were calculated. In addition, luteal cells were cultured in eight-chamber slides and treated as above, but on day 7 of culture cells were fixed and stained for the presence of 3beta-hydroxy-delta 5-steroid dehydrogenase (3beta HSD). The number of steroidogenic (3beta HSD positive) cells per unit area was counted for control cultures (no treatment) and cultures treated with the most effective doses of FGF-1, FGF-2, LH, OR FBS in proliferation and (or) progesterone assays. FGF-1, FGF-2, AND FBS stimulated (p < 0.05) proliferation of luteal cells from all stages of lu...Continue Reading

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Citations

Jun 5, 2002·Molecular and Cellular Endocrinology·Michael F SmithGeorge W Smith
Dec 18, 2002·International Journal of Experimental Pathology·Lawrence P ReynoldsDale A Redmer
Nov 25, 2003·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Kay M AbdullahAnna T Grazul-Bilska
Jun 23, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Kathryn J WoadRobert S Robinson
Nov 12, 2013·Domestic Animal Endocrinology·L A FátimaP C Papa
Aug 24, 2013·Reproduction in Domestic Animals = Zuchthygiene·D J SkarzynskiR Meidan
Mar 1, 2002·Biology of Reproduction·William A RickeMichael F Smith
Mar 22, 2001·Biology of Reproduction·L P Reynolds, D A Redmer
Mar 24, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Ewa BorowczykAnna T Grazul-Bilska
Aug 8, 2018·Endocrinology·Ketan Shrestha, Rina Meidan
Dec 7, 2018·PloS One·Sumitra DewanKris Verheyen
Nov 2, 2006·Endocrine Reviews·Carlos StoccoGeula Gibori
May 15, 2007·Endocrinology·Ann E DrummondJock K Findlay
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Jan 31, 2021·Animal : an International Journal of Animal Bioscience·K K Piotrowska-TomalaD J Skarzynski
Sep 9, 2017·Domestic Animal Endocrinology·A KraisoonA T Grazul-Bilska

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