Proliferation kinetics of perturbed cell populations determined by the bromodeoxyuridine-33258 technique: radiotoxic effects of incorporated [3H]thymidine

Cytometry
H P Beck

Abstract

A simple analytical method is described for the evaluation of flow cytometric data of perturbed cell populations obtained by applying the BrdUrd-33258 Hoechst technique. This procedure allows for determining the growth curve, the efflux from and the influx into G2 + M as well as the transit characteristics of cell cohorts through the G2 + M phase. As an example of the application of the method, the radiotoxic effects of incorporated 3H-thymidine on the proliferation of L-929 cells in vitro are described.

References

Sep 1, 1979·Cell Biophysics·J C Wood, P Tood
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S A Latt

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Citations

Jan 1, 1984·Cancer Investigation·F Traganos
Feb 1, 1988·Experimental Cell Research·P S RabinovitchH Hoehn

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