Proliferation of mouse fibroblasts induced by 1,2-dimethylhydrazine auto-oxidation: role of iron and free radicals

Biochemical and Biophysical Research Communications
M Gamberini, L C Leite

Abstract

Activation of 1,2-dimethylhydrazine (DMH) by prolonged auto-oxidation (24-h) induced proliferation of mouse fibroblasts at low hydrazine concentrations (0.1-1.0 mM) as determined by [3H-methyl]-thymidine uptake of confluent quiescent cells. Incubations were performed under conditions in which alkyl radicals are slowly formed by DMH auto-oxidation. The proliferative stimulus induced by DMH auto-oxidation complements that induced by insulin, PMA, and EGF. Inhibition by the iron chelators, o-phenanthroline and desferrioxamine, demonstrates that the induction of the proliferative effect is dependent on simple iron complexes. Proliferation was also inhibited by superoxide dismutase, catalase, and mannitol, implicating reactive oxygen species, although superoxide dismutase and catalase also inhibited alkyl radical formation, as determined by spin-trapping. These results suggest that cell proliferation induced by DMH auto-oxidation is mediated by reactive oxygen species, mainly the hydroxyl radical, and is dependent on simple iron complexes, possibly involving the Fenton reaction.

References

Jan 1, 1977·Mutation Research·R F Kimball
Jan 1, 1991·Free Radical Biology & Medicine·M A Trush, T W Kensler
Aug 31, 1990·Science·S M Cohen, L B Ellwein
May 1, 1989·Archives of Biochemistry and Biophysics·L C Leite, O Augusto
Jan 25, 1985·Science·P A Cerutti
Dec 1, 1985·Environmental Health Perspectives·B Kalyanaraman, B K Sinha
May 1, 1968·Mutation Research·E FreeseE B Freese

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Citations

Nov 1, 2007·Environmental Health and Preventive Medicine·Mizue Okada, Yoshinori Okada
Jun 28, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·M Andrea Azcárate-PerilJosé M Bruno-Bárcena
Dec 11, 2007·Journal of Medical Microbiology·Alejandra de Moreno de LeBlancFernando Sesma

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