Proliferation of rat granulosa cells during the periovulatory interval

Endocrinology
Jennifer D CannonC L Chaffin

Abstract

Granulosa cell proliferation during luteinization and terminal differentiation has historically been assumed to decline rapidly after an ovulatory stimulus. In contrast, terminal differentiation in other cell types has recently been associated with a transient increase in proliferation, suggesting that this may occur in the ovarian follicle. The goal of the current study was to test the hypothesis that an ovulatory stimulus to rats results in additional granulosa cell proliferation before cell cycle arrest. Immature rats were given a single injection of pregnant mare serum gonadotropin (PMSG) followed by human chorionic gonadotropin (hCG) to initiate periovulatory events. The proportion of granulosa cells in S phase did not change until 12 h after hCG, although the majority of the post-hCG proliferation was localized to cumulus granulosa cells for up to 10 h after hCG. The expression of cyclin D2 mRNA did not decline until 12 h after hCG, although both cyclin-dependent kinase (Cdk)4 and Cdk6 mRNA increased at 6 h. Protein levels of cyclin D2 and Cdk4 did not change as a result of hCG, whereas cyclin E increased 6 h after hCG. Kinase activity of Cdk2 dropped markedly by 4 h after hCG, but a slight increase in activity was eviden...Continue Reading

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Citations

Mar 16, 2005·Molecular and Cellular Endocrinology·Tara Lovekamp-Swan, Charles L Chaffin
Mar 12, 2009·American Journal of Physiology. Endocrinology and Metabolism·Jenna K Nyholt de PradaCatherine A VandeVoort
Mar 19, 2009·American Journal of Physiology. Endocrinology and Metabolism·Jennifer D CannonCharles L Chaffin
Jan 5, 2014·PloS One·Shin YoshiokaKiyoshi Okuda
Nov 7, 2006·Molecular and Cellular Endocrinology·Jennifer D CannonCharles L Chaffin
Sep 26, 2014·Biology of Reproduction·F Xavier DonadeuNadine Gérard
Jul 17, 2013·Experimental Biology and Medicine·Charles L Chaffin, Catherine A Vandevoort
Apr 28, 2017·Molecular Genetics and Genomics : MGG·Prashant SinghSuneel Kumar Onteru

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